Determination of Puerarin and Daidzin from Pueraria Root Based on Chromatography

Abstract:

Combine thin layer chromatography ( TLC ) with high performance liquid chromatography ( HPLC ) detection of kudzu root isoflavones, to establish a rapid and accurate method to determine the component content of Puerarin and daidzin in Radix Puerariae.Though study on the optimization analysis of Puerarin Content in Radix Puerariae and daidzin content chromatography in a variety of conditions, including TLC developing solvent and options of coloration, and HPLC chromatographic separation conditions and detection characteristics of TLC and HPL, to get the best Chromatographic separation conditions and make a comparison with Puerarin pharmacopoeia test methods.The experimental results show that: TLC has the advantages of simple operation, rapid, reproducible, rapid identification of Puerarin and daidzin , HPLC with good stability, great accuracy and high precision that can be detected in puerarin and daidzin content. TLC and HPLC is a simple, fast, accurate method and contribute to the establishment of puerarin medicine standardized quality evaluation system and comprehensive utilization and development of Pueraria resources.

• Periodical: Advanced Materials Research (Volumes 524-527)
• Pages: 2273-2277
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.524-527.2273
• Online since: May 2012
• Authors: Xing Rong Liu, Jian Zheng, Dao Chen Zhu, Yuan Mei Chen
• Keywords: Detection, High Performance Liquid Chromatography (HPLC), Pharmacopoeia, Pueraria Isoflavones, TLC

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