Detection of Silver Nanoparticles Internalization into Petunia, Petunia hybrida, Isolated Protoplasts

Abstract:

The use of nanoparticles has spannded many divisions of biological fields including sensors, drug delivery and clinical diagnosis. These functions all based on the most important action in cells called Endocytosis. The study of endocytosis in higher order plants remained inactive for a long period of time. In this work we use Silver Nano Particles (S.N.Ps) as a endocytosic substances were incubated by isolated protoplast of petunia .Concurrently, with the study of internalization, their appear aggregation inter and intracellular were investigated by Transmissions Electron Microscopy (TEM). Results and observations showed that the S.N.Ps crossed the cell membrane and internalized in to the cells. It can be assumed that the particles with the organic functionality – PEG coated – were internalized by the petunia protoplasts.

• Periodical: Advanced Materials Research (Volumes 622-623)
• Pages: 878-882
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.622-623.878
• Online since: December 2012
• Authors: Roohangiz Naderi, Sarah Jafari Rad, Aliakbar Saboor Yaraghi, Mehdi Farhoodi, Mina Nemati
• Keywords: Internalization, Petunia protoplasts, Silver Nanoparticle

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