Sequence Analysis of the Cas2 Gene in Riemerella anatipestifer

Abstract:

Objective: By analyzing the Cas2gene of Riemerella anatipestifer (RA), the aim was to acquire more useful information as a guide of further study. Methods: Using bioinformatics method, sequence analysis of RA Cas2 gene was performed with some databases and software, including homology search, sequence alignment, phylogenetic tree analysis, composition analysis and physicochemical property analysis. Results: Homology search suggested that Cas2 protein sequence of R. anatipestifer showed high similarity to those of strains within Bergeyella zoohelcum and Capnocytophaga canimorsus, consistent with the result of multiple sequence alignment and phylogenetic tree analysis. The rare codon analysis revealed that there was no rare codon string in RA Cas2 gene. Conclusion: Cas2 gene of R. anatipestifer was evolutionally closely related to those strains of Bergeyella zoohelcum and Capnocytophaga canimorsus, and can be over-expressed in E.coli without considering the impact of rare codons on translation.

• Periodical: Advanced Materials Research (Volume 647)
• Pages: 570-576
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.647.570
• Online since: January 2013
• Authors: Yang He, An Chun Cheng, Ming Shu Wang, De Kang Zhu, Xiao Jia Wang, Xin Zhang
• Keywords: Bioinformatics, Cas2 Gene, CRISPR-Cas System, Riemerella anatipestifer, Sequence Analysis

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