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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 647Development and Evaluation of a Loop-Mediated...

Development and Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Method for Detecting Foodborn Salmonella in Raw Milk

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Abstract:

In present study, we reported the performance of a Loop-mediated isothermal amplification (LAMP) assay detecting food-borne pathogen Salmonella. Three pairs of primers were specially designed for recognizing eight distinct sequences on the target invA gene. Time and temperature conditions for amplification of Salmonella were optimized to be 40 min at 61°C. The LAMP assay gave with artificially contaminated raw milk samples detection limit level of 142 CFU/ml which corresponds to 6-9 cells per reaction tube, while the detection level of conventional PCR was 103 CFU/ml. Data on naturally contaminated raw milk samples indicated that the LAMP method was highly specific and sensitive, giving 89.58% concordance with the ISO 6579 reference method for the samples without enrichment and 100% concordance for the samples after enrichment.

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Biomaterial and Bioengineering

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Periodical:

Advanced Materials Research (Volume 647)

Pages:

577-582

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.647.577

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Online since:

January 2013

Authors:

Yong Zhen Wang, De Guo Wang

Keywords:

Loop-Mediated Isothermal Amplification, Raw Milk, Salmonella

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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