Isolation and Mutagenesis of a Novel Phenol-Degrading Strain

Abstract:

In this study, with phenol as sole source of carbon, a phenol-degrading strain was isolated from rotting wood and polluted sludge. The strain was identified as Rhodococcus ruber SD3 according to their morphological features and 16S rDNA sequence. Rhodococcus ruber SD3 almost completely degraded 1.0g L-1 phenol in 72 hours. Rhodococcus ruber SD3 was also capable of growing in a medium containing isooctane, cyclohexane, benzene, n-heptane, toluene, acetonitrile, chlorobenzene, naphthalene, n-hexane, 1-naphthol and dimethylbenzene as sole carbon source, respectively. Rhodococcus ruber SD3 was mutated using LiCl as a chemical mutagen. The optimal concentration of LiCl for mutagenesis was 0.3 %. The mutant M1 could degrade 99.8 % of 1.5 g L-1 phenol within 72h. The optimum pH and temperature for the degradation of phenol with mutant M1 were 7.5 and 35°C.

• Periodical: Advanced Materials Research (Volume 647)
• Pages: 588-594
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.647.588
• Online since: January 2013
• Authors: Ren Peng, Gui Juan Yang, Qi Ming Wang, Yun Yun Du, Jia Rong Li
• Keywords: Biodegradation, Chemical Mutagenesis, Phenol, Rhodococcus ruber

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