Real-Time Investigation of Engineered Nanomaterials Cytotoxicity in Living Alveolar Epithelia with Hopping Probe Ion Conductance Microscopy
Widely used engineered nanomaterials (NMs) display unique properties that may have impact on human health, and thus require a reliable evaluation of their potential cytotoxicity. There is a continuing need for real-time imaging techniques capable of studying the interactions between NMs and living alveolar epithelial cells under physiological conditions. A new developed noninvasive HPICM is designed for continuous high-resolution topographic imaging of living cells, which makes it an ideal tool to study NMs cytotoxicity in living alveolar epithelia by performing reliable repetitive scanning. In this review, we concisely introduced the operation principle of HPICM and its applications to real-time investigation of engineered NMs cytotoxicity in living alveolar epithelia. Published results demonstrate that non-contact HPICM combined with patch-clamp has the potential to become a powerful microscopy for real-time studies of NM-cell interactions under physiological conditions.
X. Liu et al., "Real-Time Investigation of Engineered Nanomaterials Cytotoxicity in Living Alveolar Epithelia with Hopping Probe Ion Conductance Microscopy", Advanced Materials Research, Vol. 651, pp. 24-28, 2013