Response Surface Methodology for Optimization of the Ultrasonic Extraction of Polysaccharides from Melaleuca ahemifolia

Abstract:

An ultrasonic-assisted procedure for the extraction of polysaccharides from Melaleuca ahemifolia was investigated using response surface methodology (RSM). A Box–Behnken design (BBD) was employed to investigate the effects of extraction time, ratio of water to raw material and ultrasonic power on the extraction yield of polysaccharides.The statistical analysis indicated that three variables and the quadratic terms had significant effects on the yield (p＜0.05). The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and further analyzed by appropriate statistical methods (ANOVA). The optimum extraction conditions were as follows: extraction time of 21 min, ratio of water to raw material of 40, ultrasonic power of 170 W. Under the optimization conditions, the experimental yield of polysaccharides was 6.194%, which was in good agreement with the predicted value (6.288%).

• Periodical: Advanced Materials Research (Volumes 655-657)
• Pages: 1987-1995
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.655-657.1987
• Online since: January 2013
• Authors: Yue Xiao, He Ping Yu, Li Jing Lin
• Keywords: Melaleuca Ahemifolia, Polysaccharide, Response Surface Methodology (RSM), Ultrasonic Extraction

References

[1] Yang B, Jiang YM, Shi J, et al. Extraction and pharmacological properties of bioactive compounds from longan (Dimocarpus logan lour. ) fruit-A review [J]. Food Research International, 2010, 44: 1837-1842.

DOI: 10.1016/j.foodres.2010.10.019

[2] Yu J, Cui PJ, Zeng WL, et al. Protective effect of selenium-polysaccharides from the mycelia of Coprinus comatus on alloxan-induced oxidative stress in mice [J]. Food Chemistry, 2009, 117: 42-47.

DOI: 10.1016/j.foodchem.2009.03.073

[3] Hemwimon S, Pavasant P, Shotipruk A. Microwave assisted extraction of antioxidative anthraquinones from roots of Morinda cirifolia [J]. Separation and Purification Technology, 2007, 54: 44-50.

DOI: 10.1016/j.seppur.2006.08.014

[4] Hofmann R, Kappler T, Posten C. Pilot scale press electrofiltration of biopolymers [J]. Separation and Purification Technology, 2006, 51: 303-309.

DOI: 10.1016/j.seppur.2006.01.015

[5] Hromadkova Z, Ebringerova A. Ultrasonic extraction of plant materials Investigation of hemicelluloses release from buckwheat hulls [J]. Ultrasonic Sonochemistry, 2003, 10: 127-133.

DOI: 10.1016/s1350-4177(03)00094-4

[6] Hromadkova Z, Ebringerova A, Valachovic P. Comparison of classical and ultrasound assisted extraction of polysaccharides from Salvia officinalis L [J]. Ultrasonic Sonochemistry, 1999, 5: 163-168.

DOI: 10.1016/s1350-4177(98)00046-7

[7] Wang YJ, Cheng Z, Mao JW, et al. Optimization of ultrasonic assisted extraction process of Poria cocos polysaccharides by response surface methodology [J]. Carbohydrate Polymers, 2009, 77: 713-717.

DOI: 10.1016/j.carbpol.2009.02.011

[8] Tsochatzidis N A, Guiraud P, Wilhelm A M, et al. Determination of velocity, size and concentration of ultrasonic cavitation bubbles by the phase-Doppler technique[J]. Chemical Engineering Science, 2001, 56: 1831-1840.

DOI: 10.1016/s0009-2509(00)00460-7

[9] Giovanni M. Response surface methodology and product optimization [J]. Food Technology, 1983, 37: 41-45.

[10] Cacace J E, Mazza G. Optimization of extraction of anthocyanins from black currants with aqueous [J]. Journal of Food Science, 2003, 68: 240-248.

DOI: 10.1111/j.1365-2621.2003.tb14146.x

[11] Ferreira S L C, Bruns R E, Ferreira H S, et al. Box–Behnken design: An alternative for the optimization of analytical methods[J]. Analytica Chimica Acta, 2007, 597: 179–186.

DOI: 10.1016/j.aca.2007.07.011

[12] Muralidhar R V, Chirumamilla R R, Ramachandran V N, et al. Racemic resolution of RS-baclofen using lipase from Candida cylindracea[J]. Mededelingen, 2001, 66: 227–232.

[13] Atkinson G L, Donev A N. Optimum Experimental Design [M]. Oxford: Clarendon Press.

[14] Cai W R., Gu X H., Tang J. Extraction, purification, and characterization of the polysaccharides from Opuntia milpa alta [J]. Carbohydrate Polymers, 2008, 71: 403–410.

DOI: 10.1016/j.carbpol.2007.06.008

[15] Ravikumar K., Ramalingam S, Krishnan S, et al. Application of response surface methodology to optimize the process variables for reactive red and acid brown dye removal using a novel absorbent[J]. Dyes and Pigments, 2006, 70: 18–26.

DOI: 10.1016/j.dyepig.2005.02.004

[16] Muralidhar R V, Chirumamilla R R, Ramachandran V N, et al. Racemic resolution of RS-baclofen using lipase from Candida cylindracea[J]. Mededelingen, 2001, 66: 227–232.