Preparation of Chitosan/TPP Nanoparticles Used as Carrier in Enzyme Immobilization

Bi Ying Sha; Qing Shan Liu; Jing Jing You; Xiao Dan Ren; Hui Feng; Xiao Ying Yin

doi:10.4028/www.scientific.net/AMR.718-720.271

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Preparation of Chitosan/TPP Nanoparticles Used as Carrier in Enzyme Immobilization
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 718-720Preparation of Chitosan/TPP Nanoparticles Used as...

Preparation of Chitosan/TPP Nanoparticles Used as Carrier in Enzyme Immobilization

Abstract:

This work investigated the preparation of chitosan nanoparticles used as carriers for immobilized enzyme. Methods The nanoparticles were prepared by ionotropic gelation method. Response surface methodology was used in the preparation of nanoparticles. The morphologic characterization of chitosan nanoparticles was evaluated by scanning electron microscope and atomic force microscope. Results Using an appropriate experimental design, we have studied the significant influence factors (pH of the solution, concentrations of the chitosan , ratios of components) in the preparation of chitosan/triphosphate (TPP) nanoparticles.

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Periodical:

Advanced Materials Research (Volumes 718-720)

Pages:

271-274

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.718-720.271

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Online since:

July 2013

Authors:

Bi Ying Sha, Qing Shan Liu, Jing Jing You, Xiao Dan Ren, Hui Feng, Xiao Ying Yin

Keywords:

Alpha Glycosidase Enzymes, Chitosan, Immobilisation, Ionotropic Gelation Method, Nanoparticle

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