Proteomic Analysis of Gill Mucus from Grass Carp Ctenopharyngodon idella

Abstract:

Fish gills is part of the first-line defense in fish. The proteome in gill mucus of grass carp (Ctenopharyngodon idella) was examined in this study. Two-dimensional polyacrylamide gel electrophoresis, in combination with size exclusion chromatography, was used to resolve the proteins. Two-dimensional maps of gill mucus revealed 406 distinct spots with Coomassie blue staining, of which 256 protein spots were subjected to identification using matrix-assisted laser desorption / ionization time-of-flight (MALDI-TOF), matrix-assisted laser desorption / ionization tandem mass spectrometry (MALDI-MS / MS) and liquid chromatography tandem mass spectrometry (LC-MS / MS). A total of 99 spots representing 52 proteins were identified, including 19 immune-related molecules such as lectin, interlectin, transferrin, and proteasome. Their presence in gill mucus may imply the importance of non-specific immunity in gill mucus. The present study provides the first picture on proteome in mucus of a fish species, and thus the foundation for understanding quantitative and qualitative variation in proteins in the mucus.

• Periodical: Advanced Materials Research (Volumes 718-720)
• Pages: 288-292
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.718-720.288
• Online since: July 2013
• Authors: Guo Yong Liu, Xiao Tao Shi, Zhi Ying Tu, Li Ming Liu, Ying Ping Huang
• Keywords: Ctenopharyngodon idella, Gill Mucus, Grass Carp, Proteome

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