The Research on Deproteinized Techniques of Bio-Calcium Chitin

Abstract:

0Objective :This study is to retain the biological calcium in the crab shell, stripped of protein, calcium biological chitin chitin that is retained characteristics have unique biological calcium carbonate mosaic structure. the traditional preparation of chitin commoly adopts acid to remove calcium carbonae,applys alkali to extract protein, while inorgaic calcium salts is mixed with it as a source of calcium or an enhancement.Methods :Superfine pulverizing technology is used to smash crab shell to D50, which is 8.05 μm to get different size of crab shell powder through screen separation. The primary element of crab shell is calcium carbonate, chitin as well as protein. The content of protein is a significant indicator of industrial application of crab shell powder. Different size of crab shell powder is processed deproteinizedly,through hot alkali methodenzyme hydrolysis methodhot alkali method assisting microwave respectively. Results: Research result shows that the lower sizes of crab shell powder induce the higher effect of deproteinized processing method. The protein residual amount in crab shell powder of particle size D50 of 8.05 μm being processed in hot alkali for 3 hours is 0.097 %, while time being processed by microwave assisting is significantly reduced, making 20 minutes possible for the same result. Conclusions: Particle size of crab shell powder after superfine pulverizing is small, and deproteinization can be achieved by various methods, while retaining original chitin and biological calcium.

• Periodical: Advanced Materials Research (Volume 749)
• Pages: 160-166
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.749.160
• Online since: August 2013
• Authors: Rui Jie Zhang, Chang Ren Zhou, Li Hua Li, Ming Xian Liu
• Keywords: Crab Shell Powder, Deproteinized, Enzyme Hydrolysis, Microwave Reaction, Superfine Pulverizing Technology

References

[1] Fang Xu-bo, Chen Xiao-e, Yu Hui. Study on processing technology of ultra micro crab shells powder and their physico-chemical properties [J]. Food Science and Technology, 2009, 34(2): 96-98.

[2] Ma Jun-yang, Chen Yi-ping, The hemostatic mechanism and applications of chitin/chitosan [J]. Beijing Biomedical Engineering, 2007，26（04）: 442-445.

[3] Li Run-ming; Deng Shun-zhou. Progress on Application of Chitin and Chitosan in Medical Field[J]. Food and Drug 2007, 09 (12): 50-53.

[4] He Hua-ling; Yu Zhi-cai. Chitin and Chitosan: Progress of Application and Problems [J]. HE BEI FANGZHI, 2008, 4(04): 14-19.

[5] Jiang Ting-da. The research of Chitin and chitosan and byproduct utilization[J]. Fisheries Science, 1997, 16(05): 31-33.

[6] Hong Peng-zhi, Zhang Chao-hua, Guo Jia-hui. Extracting Activated Ca from Oyster Shell [J]. Journal of Zhanjiang Ocean University, 2000, 2000, 20(04): 46-49.

[7] Duplat D, Gallet M, Berland S, et al. The effect of molecules in mother-of-pearl on the decrease in bone resorption through the inhibition of osteoclast cathepsin K [J]. Biomaterials, 2007, 28 (32): 4769-78.

DOI: 10.1016/j.biomaterials.2007.07.036

[8] Vlyssides A G, Karlis P K. Thermal-alkaline solubilization of waste activated sludge as a pre-treat ment stage for anaerobic digestion[J]. Bioresource Technology, 2004, 91: 201-206.

DOI: 10.1016/s0960-8524(03)00176-7

[9] Ren Li-e, Huang Guang-rong, Jiang Jia-xin. Preparation of Ca-binding Peptides from Shrimp Heads and Shells by Enzymatic Hydrolysis [J]. Fisheries Science, 2011, 20(02): 107-111.

[10] Huang Xiao-liang; Liu Yan, et al. A Brief Review of Microwave-Assisted Protein Hydrolysis [J]. Journal of Liaoning University of Petroleum & Chemical Technology, 2006, 22(04): 53-55.

[11] Zhang Ai-Mei, Wang Rong, et al. Summarization on the Methodology Study of Protein Detection [J]. Letters in Biotechnology, 2011, 22 (01): 130-135.

[12] Zheng Xu, Wang Li-fang, Chen Fang. Evaluation on cytotoxicity of four medical materials by MTT-assay [J]. Northwest Pharmaceutical Journal, 2011, 26(05): 363-364.