Construction of Eukaryotic Expression Vector and Transient Expression on Mycobacterium bovis mpb83,mpb70 and Cattle il-15 Gene

Abstract:

Based on the splicing by overlapping extension (SOE) polymerase chain reaction (PCR),fusion gene mpb83-70 and il-15 were ligated and cloned into pMD18-T,then recombinant plasmid pMD-83-70-15 was constructed. pMD-83-70-15 and pVAX1-BMS were digested by double enzymes HindIII and EcoRI, the purified mpb83-70-il-15 was subcloned into pVAX1-BMS,then recombinant plasmid pVAX1-BMS-83-70-15 was constructed and transient expressed in Mark145 cell. These results laid solid foundations for further studies on mpb83-70-il-15.

• Periodical: Advanced Materials Research (Volume 749)
• Pages: 177-181
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.749.177
• Online since: August 2013
• Authors: Yan Ru Zheng, Chun Fang Wang, Yun Hang Gao, Jia Ning Guan, Jia Ming Lin, Da Ming Gao, Hong Xia Ma, Shuang Hou, Xiu Yun Jiang
• Keywords: Cattle il-15 Gene Eukaryotic Expression, Fusion Gene, mpb70 Gene, mpb83 Gene, Mycobacterium bovis

References

[1] S. Tollefsen, M. Vordermeier, I. Olsen, A. K. Storset, L. J. Reitan, D. Clifford, D. B. Lowrie, H. G. Wiker, K. Huygen, G. Hewinson, I. Mathiesen and T. E. Tjelle: Scand. J. Immunol. Vol. 57(2003), p.229.

DOI: 10.1046/j.1365-3083.2003.01218.x

[2] G. A. Colditz, T. F. Brewer, C. S. Berkey, M. E. Wilson, E. Burdick, H.V. Fineberg and F. Mosteller: JAMA Vol. 271(1994), p.698.

[3] H. G. Wiker, S. Nagai, R. G. Hewinson, W. P. Russell and M. Harboe: Scand. J. Immunol. Vol. 43(1996), p.374.

[4] M. Harboe, H. G. Wiker, G. Ulvund, B. Lund-Pedersen, A. B. Andersen, R. G. Hewinson and S. Nagai: Infect. Immun. Vol. 66 (1998), p.289.

DOI: 10.1128/iai.66.1.289-296.1998

[5] H. G. Wiker, K. P. Lyashchenko, A. Murat Aksoy, K. A. Lightbody, J. M. Pollock, S. V. Komissarenko, S. O. Bobrovnik, I. N. Kolesnikova, L. O. Mykhalsky, M. L. Gennaro and M. Harboe: Infect. Immun. Vol. 66 (1998), p.1445.

DOI: 10.1128/iai.66.4.1445-1452.1998

[6] H. M. Vordermeier, P. J. Cockle, A. O. Whelan, S. Rhodes, M.A. Chambers, D. Clifford, K. Huygen, R. Tascon, D. Lowrie, M. J. Colston and R.G. Hewinson: Vaccine Vol. 19(2000), p.1246.

[7] M. A. Chambers, A. Williams, G. Hatch, D. Gavier-Widén, G. Hall, K. Huygen, D. Lowrie, P. D. Marsh and R.G. Hewinson: Infect. Immun. Vol. 70 (2002), p.2159.

DOI: 10.1128/iai.70.4.2159-2165.2002

[8] M. A. Chambers, H. Vordermeier, A. Whelan, N. Commander, R. Tascon, D. Lowrie and R. G. Hewinson: Clin. Infect. Dis. Vol. 30 (2000), p. S283.

DOI: 10.1086/313875

[9] T. L. Van Belle, H. Dooms, T. Boonefaes, X.Q. Wei, G. Leclercq and J. Grooten: PLoS One Vol. 7(2012), p. e45299.

DOI: 10.1371/journal.pone.0045299

[10] W. Niedbala, X. Wei and F.Y. Liew: Eur. J. Immunol. Vol. 32(2002), p.341.

[11] M. Berard, K. Brandt, S. Bulfone-Paus and D.F. Tough:J. Immunol. Vol. 170(2003), p.5018.

[12] M.C. Ochoa, J. Fioravanti, E.H. Duitman, J. Medina-Echeverz, A. Palazon, A. Arina, J. Dubrot, C. Alfaro, A. Morales-Kastresana, O. Murillo, S. Hervas-Stubbs, J. Prieto, P. Berraondo and I. Melero: PloS One Vol. 7(2012), p. e52370.

DOI: 10.1371/journal.pone.0052370

[13] G. Leclercq, V. Debacker, M. de Smedt and J. Plum: J. Exp. Med. Vol. 184(1996), p.325.

[14] T. A. Waldmann and Y. Tagaya: Annu. Rev. Immunol. Vol. 17(1999), p.19.

[15] M. M. Sandau, J. E. Kohlmeier, D. L. Woodland and S.C. Jameson:J. Immunol. Vol. 184(2010), p.35.

[16] M. P. Rubinstein, N. A. Lind, J. F. Purton, P. Filippou, J. A. Best, P. A. McGhee, C. D. Surh and A.W. Goldrath: Blood Vol. 112(2008), p.3704.

DOI: 10.1182/blood-2008-06-160945

[17] J. Lu, R. L. 2nd Giuntoli, R. Omiya, H. Kobayashi, R. Kennedy and E. Celis: Clin. Cancer Res. Vol. 8(2002), p.3877.

[18] D. Jullien, P.A. Sieling, K. Uyemura, N. D. Mar, T. H. Rea and R. L. Modlin: J. Immunol. Vol. 158(1997), p.800.

[19] A. C. Maue, W. Ray Waters, M. V. Palmer, B. J. Nonnecke, F. C. Minion, W. C. Brown, J. Norimine, M. R. Foote, C. F. Scherer and D. M. Estes: Vaccine Vol. 25(2007), p.4735.

DOI: 10.1016/j.vaccine.2007.03.052

[20] C. Tang , H. Yamada, K. Shibata, N. Maeda, S. Yoshida, W. Wajjwalku, N. Ohara, T. Yamada, T. Kinoshita and Y. Yoshikai:J. Infect. Dis. Vol. 197(2008), p.1263.

DOI: 10.1086/586902

[21] C. Aagaard, T. T. K. T. Hoang, A. Izzo, R. Billeskov, J. Troudt, K. Arnett, A. Keyser, T. Elvang, P. Andersen and J. Dietrich: PLoS One Vol. 4(2009), p. e5930.

DOI: 10.1371/journal.pone.0005930

[22] J. L. Wang, Y. Q. Qie, B. D. Zhu, H. M. Zhang, Y. Xu, Q. Z. Wang, J. Z. Chen, W. Liu, H. H. Wang: Med. Microbiol. Immunol. Vol. 198(2009), p.5.

[23] Y. Q. Qie, J. L. Wang, W. Liu, H. Shen, J. Z. Chen, B. D. Zhu, Y. Xu, X. L. Zhang and H. H. Wang: Scand. J. Immunol. Vol. 69(2009), p.342.

[24] N. A. Parlane, D. Neil Wedlock, B. M. Buddle, and B. H. A. Rehm: Appl. Environ. Microbiol. Vol. 75(2009), p.7739.

[25] N. A. Parlane, K. Grage, J. Mifune, R. J. Basaraba, D. Neil Wedlock, B. H. A. Rehm and B.M. Buddle: Clin. Vaccine Immunol. Vol. 19(2012), p.37.

DOI: 10.1128/cvi.05505-11

[26] S. L. Young, L. J. Slobbe, M. Peacey, S. C. Gilbert, B. M. Buddle, G. W. de Lisle and G. S. Buchan: Immunol. Cell Biol. Vol. 88(2010), p.651.

DOI: 10.1038/icb.2010.25

[27] B. Abel, M. Tameris, N. Mansoor, S. Gelderbloem, J. Hughes, D. Abrahams, L. Makhethe, M. Erasmus, M. de Kock, L. van der Merwe, A. Hawkridge, A. Veldsman, M. Hatherill, G. Schirru, M. G. Pau, J. Hendriks, G. J. Weverling, J. Goudsmit, D. Sizemore, J. B. McClain, M. Goetz, J. Gearhart, H. Mahomed, G. D. Hussey, J. C. Sadoff and W. A. Hanekom: Am. J. Respir. Crit. Care Med. Vol. 181(2010).

DOI: 10.1164/rccm.200910-1484oc

[28] D. F. Hoft, A. Blazevic, J. Stanley, B. Landry, D. Sizemore, E. Kpamegan, J. Gearhart, A. Scott, S. Kik, M. G. Pau, J. Goudsmit, J. B. McClain and J. Sadoff: Vaccine Vol. 30(2012), p. (2098).

DOI: 10.1016/j.vaccine.2012.01.048