Mechanism of Ginsenoside Rh2 Inhibit Non-Small Cell Lung Cancer

Abstract:

To study function and relativity of autophagy in NSCLC treated with ginsenoside Rh2, and to explore some molecular mechanism that ginsenoside Rh2 induces A549 cell lines of NSCLC apoptosis. Methods: Immunofluorescence assays detects activity of caspase-8 / 9 and expression of autophagy-related molecules Beclin-1 and LC3. Results: Immunofluorescence assays shows that, 20μg/mL ginsenoside Rh2 acts 3h, fluorescent intensity that marks activity of caspase-8 and expression level of Beclin-1 significantly increases, it means that activity of caspase-8 significantly rises and expression level of Beclin-1 significantly increases. Fluorescent intensity that marks expression of LC3and activity of caspase-9 has increase but without significant change in 1-6h, it means expression of LC3 and activity of caspase-9 has no significant change. Conclusion: Ginsenoside Rh2 can adjust expression of membrane receptor pathway (caspase-8) and or autophagy related gene Beclin-1,then play the biological role of promoting apoptosis of tumor cell.

• Periodical: Advanced Materials Research (Volume 749)
• Pages: 167-171
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.749.167
• Online since: August 2013
• Authors: Han Gao, Yan Sun, Hai Tao Yu, Chun Jing Zhang
• Keywords: 20 (S) - Ginsenoside Rh2, A549 Cells, Apoptosis, Autophagy

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