Optimization of Nitrite Reductase Production Conditions in Lactobacillus plantarum from Salted Fish

Abstract:

The conditions for nitrite reductase (NiRs) produced by Lactobacillus plantarum from salted fish were optimized. Enzyme activity of nitrite reductase was used as evaluation index. The results showed that the pH value had greatest influence on enzyme activity. The optimum conditions of producing nitrite reductase were inoculate quantity 4 %, pH 6.3, culture temperature 35 °C, incubation time 48 h. The enzyme activity of nitrite reductase reached to 89.672 U/mL, which increased by 71 % after optimization. This work provided the foundation for the research of enzymatic properties of nitrite reductase. This may be useful to the production of salted fish products.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 1595-1598
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.1595
• Online since: September 2013
• Authors: Ya Nan Wang, Yan Yan Wu, Xian Qing Yang, Lai Hao Li, Bo Qi
• Keywords: Lactobacillus plantarum, Nitrite, Nitrite Reductase, Salted Fish

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