Determination of Fourteen Sulfonamides Residues in Penaeus vannamei by High Performance Liquid Chromatography Coupled with Post-Column Derivation

Abstract:

The method was established to detect fourteen sulfonamides residuces in Penaeus vannamei by high performance liquid chromatography coupled with post-column derivation. Sulfonamides residues were extracted with ethyl acetate after adding sulfapyridine as internal standard. The extract was concentrated.The residues were transferred to hydrochloric acid solution. The solution was defatted with n-hexane. The compounds were detected by HPLC with fluorescence detector .The standard addition method was used. The calibration curves were linear. The recoveries ranged from 77.8% to 103.6%. The relative standard deviations were all below 9.1%. Quantitative detection limits of fourteen sulfanomides ranged from1.0μg/kg to 5.0μg/kg. Results indicated that the method was easier, faster and more accurate.

• Periodical: Advanced Materials Research (Volumes 781-784)
• Pages: 903-907
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.781-784.903
• Online since: September 2013
• Authors: Dong Mei Huang, Jie Xu, Yong Fu Shi, Xuan Yun Huang, Huan Yu, Bing Feng, Yuan Wang, You Qiong Cai*
• Keywords: High Performance Liquid Chromatography (HPLC), Penaeus vannamei, Post-Column Derivation, Residuce Detection, Sulfonamide

References

[1] Bai L L, Dai H and Lin Z F: Drug identification Vol. 30 (2011), pp.60-61. (in Chinese).

[2] Zhang H Q, Song L L and Xu Q L: Journal of Analytical Chemistry Vol. 35 (2007), pp.268-272. (in Chinese).

[3] Li Z Q, Ni M L and Yu X J: Journal of Instrumental Analysis Vol. 26( 2007) , pp.508-510. (in Chinese).

[4] Liu C Z, Ai X H and Wu G: Freshwater Fisheries Vol. 34(2004) , pp.23-24. (in Chinese).

[5] L. Verzegnassi*, M. -C. Savoy-Perroud, R.H. Stadler: Journal of Chromatography A, Vol. 977(2002) , pp.77-87.

[6] So-Young Won, PranjalChandra, TakSeongHee: Biosensors and Bioelectronics , Vol. 39 (2013) , pp.204-209.

[7] Lei Sun, Ligang Chen, Xin Sun: Chemosphere Vol. 77 (2009) , p.1306–1312.

[8] Barbara Chiavarino*, Maria Elisa Crestoni, Annito Di Marzio: Journal of Chromatography B, Vol. 706( 1998) , p.269–277.

[9] Rodrigo H.M.M. Granja*, Andreia C. deLima, Alessandro G. Salerno: Food Control, Vol. 28 (2012) , p.304–308.

[10] Theresa A. Gehring*, Bill Griffin, Rod Williams : Journal of Chromatography B, Vol. 706 (1998) , p.269–277.