PU/hydrogel compounds prepared byγ-ray radiation technique were investigated in this study. We used nanosized clay solution or powder, Polyurethane (PU), thermosensitive N-isopropyl acrylamide (NIPAAm) and acrylic acid (AAc) as starting materials to prepare PU/hydrogel nanocomposite products. The dipping and weighing tests, Material Testing Machine (LLOYD) were utilized to analyze the physical and mechanical properties differentially. In addition, the drug release and wound closure experiments were also finished by Enzyme-link immunosorbent assay (ELISA) reader and five groups of treatment dressings. From the final results, We obtained that the PU/hydrogel composite incorporating FGF-2 as a wound dressing was able to effectively interact with the wound in a suitable moist healing environment. These composites make them more compatible with skin.