Extraction of Polyphenols from Porphyra and Scavenging Activity of Oxygen Free Radicals

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Abstract:

Polyphenols from Porphyra are important biological active substances. The effects of temperature, times, ethanol concentration and liquid to solid ratio were studied for the extraction yield of polyphenols from Porphyra through a single-factor exploration.Then, through an orthogonal experiment, it was investigated to get the best extraction conditions. The content of polyphenols was determined by Folin-Ciocalteu method. Scavenging ability to oxygen free radicals was also assessed. The results show that extraction temperature is 75°C, the extraction time is 2.5 h, ethanol concentration is 70% and the ratio of liquid to solid is 25:1(mL/g).The proportion of the extraction of the polyphenols from Porphyra under these industrial process conditions is 6.263mg/g. Polyphenols from Porphyra have strong scavenging hydroxyl radical and superoxide anion radical. IC50 of polyphenols from Porphyra on hydroxyl radical and superoxide anion radical is 0.405 mg/mL, 0.539mg/mL, respectively.

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Advanced Materials Research (Volumes 864-867)

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531-535

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December 2013

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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[1] G. M. Williams and A. M. Jeffrey. Oxidative DNA damage: Endogenous and chemically induced. Regulatory Toxicology and Pharmacology, Vol. 32 (2000), pp.283-292.

DOI: 10.1006/rtph.2000.1433

Google Scholar

[2] W.K. Jung, I. Choi, S. Oh, S.G. Park and I.W. Choi . Anti-asthmatic effect of marine red alga (Laurencia undulata) polyphenolic extracts in a murine model of asthma [J]. Food and Chemical Toxicology, Vol. 47 (2008): pp.293-297.

DOI: 10.1016/j.fct.2008.11.012

Google Scholar

[3] V.L. Singleton, R. Orthofer and R.M. Lamuela-Raventos. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology , Vol. 299 (1999), pp.152-178.

DOI: 10.1016/s0076-6879(99)99017-1

Google Scholar

[4] G. C. Yen and H.Y. Chen. J. Agric. Food Chem. Vol. 43(1995), pp.27-32.

Google Scholar

[5] L. Y. Chen, X. J. Meng and W. Xia. Food Science. Vol. 25(2004), pp.167-170 (In Chinese).

Google Scholar

[6] X. Duan, G. Wu and Y. Jiang. Evaluation of the antioxidant properties of litchi fruit phenolics in relation to pericarp browning prevention. Molecules. Vol. 12 (2007), pp.759-771.

DOI: 10.3390/12040759

Google Scholar