Construction and Functional Analysis of Luciferase Reporter Plasmid Containing NF-H Gene Promoter

Tong Cun Zhang; Yao Meng; Nan Wang; Feng Lin; Tao Qin; Zhe Sun; Man Li

doi:10.4028/www.scientific.net/AMR.915-916.942

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 915-916Construction and Functional Analysis of Luciferase...

Construction and Functional Analysis of Luciferase Reporter Plasmid Containing NF-H Gene Promoter

Abstract:

NF-H (a member of neurofilaments) is a protein widely expressed in all kinds of neurons after birth and can be used as one of the symbols of mature neurons. Constuction of NF-H promoter luciferase reporter plasmid will provide the theory basis for researching the effect of other transcription factors on regulating NF-H transcription. Here, human NF-H promoter luciferase reporter plasmid were successfully constructed. Then the effects of some key transcription factors were investigated by luciferase reporter assays in COS-7 cells. The results showed that retinoid X receptor α (RXRα) can enhance transcriptional activity of NF-H. Furthermore, ERK 1/2 (extracellular signal-regulated kinase 1/2) and STAT3 (signal transducer and activator of transcription 3) also show obvious impact in activating NF-H transcription.

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Periodical:

Advanced Materials Research (Volumes 915-916)

Pages:

942-946

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.915-916.942

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Online since:

April 2014

Authors:

Tong Cun Zhang*, Yao Meng, Nan Wang, Feng Lin, Tao Qin, Zhe Sun, Man Li

Keywords:

ERK1/2, Luciferase Activity Assay, NF-H, RXRα, STAT3

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References

[1] PM Steinert and DR Roop. Molecular and cellular biology of intermediate filaments[J]. Annu Rev Biochem, 1988, 57: 593-625.

DOI: 10.1146/annurev.bi.57.070188.003113

Google Scholar

[2] Malik S, Roeder RG. Transcriptional regulation through Mediator-like coactivators in yeast and metazoan cells[J]. Trends Biochem Sci. 2000, 25(6): 277-283.

DOI: 10.1016/s0968-0004(00)01596-6

Google Scholar

[3] Szanto A, Narkar V, Shen Q, Uray IP, Davies PJ, Nagy L. Retinoid X receptors: X-ploring their (patho) physiological functions[J]. Cell Death Differ, 2004, 11(2): S126-143.

DOI: 10.1038/sj.cdd.4401533

Google Scholar

[4] Gary L. J, Razvan L. Mitogen-Activated Protein Kinase Pathways Mediated by ERK, JNK, and p.38 Protein Kinases. Science, 2002, 298: 1911-(1912).

DOI: 10.1126/science.1072682

Google Scholar

[5] Lai CF, Chaudhary L, Fausto A, Halstead LR, Ory DS, Avioli LV et al. Erk is essential for growth, differentiation, integrin expression, and cell function in human osteoblastic Cells[J]. J Biol Chem, 2001, 276: 14443-144450.

DOI: 10.1074/jbc.m010021200

Google Scholar

[6] Bowman T, Yu H, Sebti S, Dalton W, Jove R. Signal Transducers and Activators of Transcription: Novel Targets for Anticancer Therapeutics. Cancer Control, 1999, 6(5): 427-435.

DOI: 10.1177/107327489900600501

Google Scholar

[7] Shih Y-Y, Lee H, Nakagawara A, Juan H-F, Jeng Y-M, Tsay YG et al. Nuclear GRP75 Binds Retinoic Acid Receptors to Promote Neuronal Differentiation of Neuroblastoma. 2011, 6(10): e26236.

DOI: 10.1371/journal.pone.0026236

Google Scholar

[8] Daniel C. Berwick，Mattia Calissano, Jacqueline D et al. Regulation Of Brn-3a N-terminal transcriptional activity by MEK1/2-ERK1/2 signalling in neural differentiation. 2009, 1256: 8-18.

DOI: 10.1016/j.brainres.2008.12.009

Google Scholar

[9] Snyder M, Huang XY, Zhang JJ. Stat3 is essential for neuronal differentiation through direct transcriptional regulation of the Sox6 gene. Oncogene, 2011, 585(1): 148-152.

DOI: 10.1016/j.febslet.2010.11.030

Google Scholar