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TEOS Hydrolysis Method Synthesize Nano Silica and Biocompatibility Research

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Abstract:

Nano silica spheres are synthesized by the TEOS hydrolysis method. The XRD pattern shows that the samples’ strongest peaks are significantly broadened. Series samples are all silica single phase. From the photos of TEM we can see that the samples’ surfaces have a good dispersion and spherical morphology. The Hela cell proliferating activity is assessed by MTT assay. The cell’s morphology and Annexin Hoechst 33342 staining are employed to determine apoptosis in Hela cells. Result shows that cytocompatibility becomes better with the lower concentrations of sample. 24h and 48h cells stained photograph shows that in the concentrations of nano silica 50μg·ml-1, the cell structure is normal and nucleus is complete.

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Periodical:

Advanced Materials Research (Volumes 915-916)

Pages:

958-961

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.915-916.958

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Online since:

April 2014

Authors:

Xin Xin Li, Zhi Long Wang*, Nin Yao, Yi Li, Yan Liang

Keywords:

Biocompatibility, Nano Silica, TEOS Hydrolysis Method

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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