Research on Antimicrobial Activity and Packaging Performance of Degradable Protein Films

Abstract:

The degradable protein film-forming materials contained 5% whey protein isolate (WPI), 2% sodium caseinate (NaCas) and 50% glycerol,which was the optimal formula obtained by uniform design method of previous work. The antimicrobial activity and packaging performance of WPI-NaCas degradable protein films were discussed by addition of nanoTiO₂ at different concentrations (0,0.05,0.10,0.15,0.20g•(200ml)^-1) in film-forming solution.The results showed that nanoTiO₂ and protein films showed a certain degree of compatibility.Addition of nanoTiO₂ could improve tensile strength and elongation at break of the protein films and decrease WVP values of the films,but decrease light transmittance,increase haze of the protein films. The degradable protein films suited for using below150°C.And,nanoTiO₂ incorporated into protein films had antimicrobial activity against both E.coli and S.aureus.

• Periodical: Advanced Materials Research (Volumes 915-916)
• Pages: 947-953
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.915-916.947
• Online since: April 2014
• Authors: Qiao Lei, Zhi Ying Huang, Jia Zhen Pan, Jian Qiang Bao, Qian Nan Xun, Shuo Jiang, Yu Ting Zhang
• Keywords: Antimicrobial Activity, Nano-TiO₂, Packaging Performance, Sodium Caseinate, Whey Protein Isolate

References

[1] Ouattara,B., Simard R.E., Piette,G., et al. Diffusion of acetic and propionic acids from chitosan-based antimicrobial packaging films[J]. Journal of Food Science, 2000, 65(5), 768–773.

DOI: 10.1111/j.1365-2621.2000.tb13584.x

[2] Siragusa G.R., Dickson J.S., Inhibition of Listeria monocytogenes on beef tissue by application of organic acids immobilized in a calcium alginate gel[J]. Journal of Food Science, 1992, 57(2), 293–296.

DOI: 10.1111/j.1365-2621.1992.tb05479.x

[3] Gennadios,A., Weller C.L., Edible films and coatings from wheat and corn proteins[J]. Food Technology, 1990, 44(10), 63–69.

[4] Rico-Pena D.C., Torres J.A., Sorbic acid and potassium sorbate permeability of an edible methylcellulose-palmitic acid films: water activity and pH effects[J]. Journal of Food Science, 1991, 56(2), 497–499.

DOI: 10.1111/j.1365-2621.1991.tb05312.x

[5] Kokoszka, S., Debeaufort, F., Lenart, A., Voilley, A. Water Vapour Permeability, Thermal and Wetting Properties of Whey Protein Isolate Based Edible Films[J]. International Dairy Journal. 2010, (1): 53-60.

DOI: 10.1016/j.idairyj.2009.07.008

[6] G.Y. Chen,Q. Lei: Research on Preparation and Properties of Edible Composite Protein Films[J]. Applied Mechanics and Manufacturing Technology, 2011, (87): 213-222.

DOI: 10.4028/www.scientific.net/amm.87.213

[7] Abugroun H.A., Cousin N.A., Judge M.D., Extended shelf life of unrefrigerated prerigor cooked meat[J]. Meat Science, 1993(33): 207–229.

DOI: 10.1016/0309-1740(93)90060-u

[8] Z.G. Yang, Preparation and antibacterial properties of nano-TiO2 particles and nanotubes[D]. Qingdao: Qufu Normal University, (2009).

[9] Schulz,J., Hohenberg,H., Pflücker,F., et al. Distribution of sunscreens on skin[J]. Advanced Drug Delivery Review. 2002, 54(1), 157-163.

DOI: 10.1016/s0169-409x(02)00120-5

[10] Tao, Y., Pan, J., Yan, S., et al. Tensile strength optimization and characterization of chitosan/TiO2 hybridfilm[J]. Materials Science and Engineering B: Solid-State Materials for Advanced Technology. 2007, 138(1), 84-89.

DOI: 10.1016/j.mseb.2006.12.013

[11] Zhou J.J., Wang, S.Y., & Gunasekaran,S. Preparation and characterization of whey protein film incorporated with TiO2 nanoparticles. Journal of Food Science, 2009, 74(7), 50-56.

[12] Ghasemlou, M, Khodaiyan, F, Oromiehie,A. Physical, mechanical, barrier, and thermal properties of polyol-plasticized biodegradable edible film made from kefiran[J]. Carbohydrate Polymers, 2011, 84(1): 477-483.

DOI: 10.1016/j.carbpol.2010.12.010

[13] ASTM, 2009. Designation: D882–09: Standard Test Method for Tensile Properties of Thin Plastic Sheeting.

[14] ASTM D1003-61, 1997: Standard Test Method for Haze and Luminous Transmittance of Transparent Plastics.

[15] ASTM E-398, 2003: Standard Test Method for Water Vapor Transmission Rate of Sheet Materials Using Dynamic Relative Humidity Measurement.

DOI: 10.1520/e0398-13

[16] C.D. Mu.L.M. Li and L. Wei et al: Preparation and properties of dialdehyde carboxyrnethyl cellulose crosslinked gelatin edible films [J]. Food Hydrocolloids, 2012, 27: 22-29.

DOI: 10.1016/j.foodhyd.2011.09.005

[17] C. Ping,K. Wang and L. Ma: Antimicrobial Activity and Solubility of Whey Protein Edible Film[J]. Meat Industry, 2012, (6): 35-37.

[18] M. Chen C.Y. Hu and Z.W. Wang et al: Effects of Vanillin on Antimicrobial Activity of Soy Protein Isolated Film[J]. Packaging Engineering, 2008, 29(10): 83-85.

[19] Y.X. Li,Y.F. Jiang and F. Liu, et al: Fabrication and characterization of TiO2/whey protein isolate nanocompositefilm[J]. Food Hydrocolloids. 2011, 25(5): 1098-1104.

DOI: 10.1016/j.foodhyd.2010.10.006

[20] M.J. Fabra, P. Talens and A. Chiralt: Journal of Food Engineering Vol. 96 (2010), pp.356-364.

[21] Hoque M.S., Benjakul,S., & Prodpran,T. (2011b). Effects of partial hydrolysis and plasticizer content on the properties of film from cuttlefish (Sepia pharaonis) skin gelatin. Food Hydrocolloids, 25, 82-90.

DOI: 10.1016/j.foodhyd.2010.05.008

[22] Martucci J.F., & Ruseckaite R.A. (2009). Tensile properties, barrier properties, and biodegradation in soil of compression-molded gelatin-dialdehyde starch films. Journal of Applied Polymer Science, 112, 2166-2178.

DOI: 10.1002/app.29695

[23] Cagri, A., Ustunol, Z., Ryser, E.T. et al. Antimicrobial, mechanical, and moisture barrier properties of low pH whey protein-based edible films containing p-Amminobenzoic or sorbic acid [J]. Journal of Food Science, 2001, 66(6), 865–870.

DOI: 10.1111/j.1365-2621.2001.tb15188.x