Optimization of Ferment Condition of ε-Poly-L-Lysine by Response Surface Methodology

Abstract:

Streptomyces albus Y07, a strain which could produce ε-Poly-L-lysine (ε-PL) had been screened in our previous study. The fermentation conditions for ε-Poly-L-lysine production were further optimized through response surface method in this work. A central composite design was adopted to study the effect of culture volume, shaking speed, initial culture pH, as well as inoculation amount on ε-PL production. A quadratic polynomial equation for ε-Poly-L-lysine production was obtained by multiple regression analysis. The equation was as follow: The accuracy and general availability of the polynomial model were proved to be adequate with R² Pre = 0.8373. The optimized conditions were as follows: culture volumn: 50/500ml, culture pH = 7, inoculation amount: 10% of the total culture volume, shaking speed: 180rpm. Under the optimized conditions, the ε-PL production reached 1.085g / L. And the validity of the predicited model was further confirmed through verification experiments..

• Periodical: Advanced Materials Research (Volume 934)
• Pages: 96-103
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.934.96
• Online since: May 2014
• Authors: Yu Hong Yang, Hao Wang*, Hong Ling Wang, Zhuo Wang, Zong Li Kang
• Keywords: Optimization, Response Surface Methodology (RSM), ε-Poly-L-Lysine

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