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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 997Isolation and Expression Analysis of a Putative...

Isolation and Expression Analysis of a Putative CIPK Gene in Halophyte Sesuvium portulacastrumand L.

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Abstract:

Calcium plays a significant role in intracellular signaling. Calcineurin B-like proteins (CBLs) and their target proteins, CBL-interacting protein kinases (CIPKs) have emerged a calcium network responsing to stresses in plants. In this study, we cloned a CIPK gene, SpCIPK8, from the halophyte plant Sesuvium portulacastrumand L. Bioinformation analysis showed that the cDNA of SpCIPK8 was 1344 bp and encoded 447 amino acids with a molecular weight of 50.72 KD and an isoelectric point of 7.19. One transmembrane domain exists in this hydrophilic protein. Phylogenetic analysis showed that SpCIPK8 was similar with AtCIPK8 in Arabidopsis.The expression level of SpCIPK8 in stems was three times higher than that in roots and the level in leaves was two times higher than that in roots.

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Frontiers of Chemical Engineering, Metallurgical Engineering and Materials III

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Periodical:

Advanced Materials Research (Volume 997)

Pages:

215-220

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.997.215

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Online since:

August 2014

Authors:

Yan Ping Hu*, Yang Zhou, Cheng Long Yang, Jiao Liu, Rui Jun Duan, Shao Ping Fu, Xin Wen Hu, Jian Chun Guo

Keywords:

Expression Pattern, Gene Cloning, Sesuvium portulacastrumand L., SpCIPK8

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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