In Situ Imaging of Cancer Cell via Cancer Aptamer and its Fluorescent Clusters

Lu Ga; Jun Ai

doi:10.4028/www.scientific.net/AMR.998-999.256

Paper Titles

Effects of Self-Assembling Peptide RADA16-І Hydrogel on Neural Differentiation of Bone Marrow Mesenchymal Stem Cells
p.238

Exercise Induce HO-1 Expression in Skeletal Muscle and Improve Sports Ability of Mice
p.243

Expression of a Fusion Protein of Human Proinsulin with Glutathione-S-Transferase in Escherichia coli
p.248

Foxp3 in Lewis Lung Cancer Cells Enhances the Expression of TGF-β1 and IL-10
p.252

In Situ Imaging of Cancer Cell via Cancer Aptamer and its Fluorescent Clusters
p.256

Inhibition of HIF-1α Signaling in the Ovaries of Sprague-Dawley Rats with Polycystic Ovary Syndrome
p.260

IRE1a Negative Regulates Chondrocyte Differentiation
p.265

Neuroprotection of Saponins from Rhizoma panacis majoris in Cerebral Ischemia/Reperfusion is Related to Nrf2-Mediated Antioxidant Response: Role of Nrf2 and Bcl-2 Family Expressions
p.269

Optimum the Fermentation Condition to Produce the Polysaccharide of Hericium erinaceus with Tofu Whey
p.275

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 998-999In Situ Imaging of Cancer Cell via Cancer Aptamer...

In Situ Imaging of Cancer Cell via Cancer Aptamer and its Fluorescent Clusters

Abstract:

Silver nanoclusters synthesized using aptamer as matrices attracted more attention in the fields of medicine, pharmacology and biology. In this paper, we report a novel approach to sgc-8-C8-templated formation of fluorescent Ag nanoclusters and its application to bioimaging. This was further confirmed by flow cytometry. The cell toxicity (3-[4, 5-dimethylthiazolyl-2]-2, 5 -diphenyltetrazolium bromide, MTT) assay demonstrated that the silver nanoclusters has only little affect on the cytotoxicity to the cells. These results demonstrated that this aptamer-based method for labeling and imaging cellular protein is facile, effective.

You might also be interested in these eBooks

Advances in Applied Sciences, Engineering and Technology II

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 998-999)

Pages:

256-259

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.998-999.256

Citation:

Cite this paper

Online since:

July 2014

Authors:

Lu Ga, Jun Ai*

Keywords:

Aptamer, Bioimaging Fluorescence, Silver Nanocluster

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] (a) T. Vosch, Y. Antoku, J. -C. Hsiang, C. I. Richards, J. I. Gonzalez, R. M. Dickson, Proc. Natl. Acad. Sci. 2007, 104, 12616 -12621. (b) J. P. Wilcoxon, B. L. Abrams, Chem. Soc. ReV. 2006, 35 (11), 1162–1194. (c) J. Zheng, P. R. Nicovich, R. M. Dickson, Annu. ReV. Phys. Chem. 2007, 58, 409–431. (d) S. Link, A. Beeby, S. FitzGerald, M. A. El-Sayed, T. G. Schaaff, R. L. Whetten, J. Phys. Chem. B 2002, 106 (13), 3410–3415.

DOI: 10.1073/pnas.0610677104

Google Scholar

[2] J.T. Petty, J. Zheng, N.V. Hud, R. M. Dickson, J. Am. Chem Soc. 2004, 126, 5207-5212.

Google Scholar

[3] (a) C.M. Ritchie, K.R. Johnsen, J. R. Kiser, Y. Antoku, R. M. Dickson, J. T. Petty, J. Phys. Chem. C 2007, 111, 175-181. (b) K. Koszinowski, K. Ballweg, Chem. Eur. J. 2010, 16, 3285 – 3290.

DOI: 10.1021/jp0648487

Google Scholar

[4] J. Yu, S. Choi, C. I. Richards, Y. Antoku, R. M. Dickson, Photochem Photobiol, 2008, 84: 1435–1439.

Google Scholar