Foxp3 in Lewis Lung Cancer Cells Enhances the Expression of TGF-β1 and IL-10

Li Song Chen; Xiao Dong Gai; Chun Li

doi:10.4028/www.scientific.net/AMR.998-999.252

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 998-999Foxp3 in Lewis Lung Cancer Cells Enhances the...

Foxp3 in Lewis Lung Cancer Cells Enhances the Expression of TGF-β1 and IL-10

Abstract:

In order to explore the related mechanism of Foxp3 in tumor immune escape, the study detected the expression of Foxp3 in lewis lung cancer (LLC) cells and analyzed the expression of TGF-β1 and IL-10 in Foxp3 overexpressed LLC cells. Foxp3 mRNA was detected in LLC cells by RT-PCR. Foxp3 was highly expressed in Foxp3 transfected LLC group than that of in empty vector group and LLC group by RT-PCR(P <0.01). The mRNA and protein expression of TGF-β1 and IL-10 significantly increased in Foxp3 transfected LLC group than that of in empty vector group and LLC group by RT-PCR and ELISA(P <0.05). These results suggest that Foxp3 in LLC cells may promote tumor immune escape by enhancing the expression of TGF-β1 and IL-10.

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Periodical:

Advanced Materials Research (Volumes 998-999)

Pages:

252-255

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.998-999.252

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Online since:

July 2014

Authors:

Li Song Chen, Xiao Dong Gai, Chun Li*

Keywords:

Foxp3, IL-10, Lewis Lung Cancer, TGF-β1, Transfection

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* - Corresponding Author

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