Effects of Self-Assembling Peptide RADA16-І Hydrogel on Neural Differentiation of Bone Marrow Mesenchymal Stem Cells

Abstract:

Objective: To establish composite culture system of rat bone marrow mesenchymal stem cells (BMSCs) and self-assembling peptide hydrogel RADA16-І, and to investigate the effect of RADA16-І hydrogel on neural differentiation of BMSCs. Methods: BMSCs were isolated, cultivated and labeled with green fluorescent protein (GFP), then they were inoculated on glass coverslips or in RADA16-І solution to form control group and RADA16-І group respectively. The morphological changes of BMSCs induced by neural induction medium were observed, and GFAP, NeuN and Map-2 expressions of BMSCs in each group were detected with immunofluorescence. Results: The induced BMSCs presented neuron-like change, and the rates of GFAP and NF-200 positive cells in RADA16-І group were higher than that in control group (P < 0.05). Conclusion: Self-assembling peptide RADA16-І hydrogel can promote neural differentiation of BMSCs, and which may be used as scaffold material on BMSCs transplantation for treatment of nervous system diseases.

• Periodical: Advanced Materials Research (Volumes 998-999)
• Pages: 238-242
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.998-999.238
• Online since: July 2014
• Authors: Yan Sheng Liao, Li Deng, Xiao Qing Gao, Chao Xian Yang*
• Keywords: Bone Marrow Mesenchymal Stem Cells, Neural Differentiation, Self-Assembling Peptide RADA16-І Hydrogel

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