Method Validation for Determination of Caffeine in Sidomulyo Robusta Coffee Beans Using Gas Chromatography-Flame Ionization Detector

Abstract:

Caffeine, an alkaloid compound found in coffee, is widely known for its ability to stimulate the human central nervous system. The caffeine content in Sidomulyo robusta coffee was analyzed using a gas chromatography-flame ionization detector (GC-FID) with ultrasound-assisted extraction as a preparation method using methanol solvent for 21 minutes at 32°C. A TR-1MS column (15m x 0.25 mm ID x 0.25 μm film, non-polar column 100% dimethyl poly-siloxane) was used for the GC analysis. The injector temperature was set at 260°C and the detector at 280°C. The initial oven temperature was set at 100°C, held for 1 minute, then gradually increased by 5°C/minute up to 280°C and held for 2 minutes, with the optimal flow rate condition selected at 1.2 mL/min. This method met all verification criteria and exhibited linearity in the 100–600 mg/L range with a high correlation coefficient of 0.9929, demonstrating its reliability and accuracy in determining caffeine content in coffee samples

• Periodical: Engineering Headway (Volume 24)
• Pages: 47-56
• DOI: https://doi.org/10.4028/p-XLxbQ8
• Online since: July 2025
• Authors: Salsabila Firdausi Zahra, Asnawati Asnawati, Zulfikar Zulfikar, Siswoyo Siswoyo, Tri Mulyono, Yeni Maulidah Muflihah*
• Keywords: Caffeine, Gas Chromatography, Robusta Coffee, Validation

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