In Vitro Evaluation of Antibacterial Properties of Biogenically Synthesized Zinc Oxide Nanoparticles on Pathogenic Paddy Bacteria

Abstract:

Zinc oxide nanoparticles (ZnO-NP) were successfully synthesized from aloe vera extract and zinc sulphate as zinc precursor. Characterizations of the ZnO-NP were performed using UV–Vis spectrophotometer, XRD, FESEM and TEM analysis. The absorption peak from UV–Vis was at 380 nm while the XRD diagram displays high purity ZnO-NP. FESEM and TEM analysis showed agglomerated particles with a wide size distribution range. Polymerase chain reaction (PCR) analysis of Xanthomonas oryzae pv. oryzae (Xoo) pathotype 0.0 generated a product with the size of 230 bp similar to in silico PCR results, verifying the pathotype on molecular level. Subsequently, the antimicrobial activities of the ZnO-NP against Xoo pathotype 0.0 were assessed. Xoo (10⁸ cfu/ml) were grown in LB broth supplemented with various concentrations of ZnO-NP. Collection of samples were done at 24 hours, 48 hours and 72 hours of incubation, grown on LB agar and observed for bacterial growth. Colony forming unit (cfu/ml) values revealed the number of viable cells decreased with high concentrations of ZnO-NP whereas minimal inhibition was observed at lower ZnO-NP concentrations. At lower bacteria cfu/ml (10³ cfu/ml), it was found that at 24 hours incubation, ZnO-NP gave comparable antibacterial effects to commercial ZnO-NP and commercial non-nanoZnO after exposure for 1 hour. However, the antimicrobial effects decreased after 48 hours. It was also noted that the ZnO-NP provide better suppression of bacterial growth at lower bacterial concentration.

• Periodical: Journal of Biomimetics, Biomaterials and Biomedical Engineering (Volume 59)
• Pages: 1-10
• DOI: https://doi.org/10.4028/p-cu9pvj
• Online since: February 2023
• Authors: Rafidah Abd Rahman*, Chin Hua Chia, Noor Azlina Masdor
• Keywords: Anti-Bacteria, Nanomaterial, Rice, Xanthomonas oryzae Pv. Oryzae, Zinc Oxide

References

[1] I. Khan, K. Saeed, I. Khan, Nanoparticles: Properties, applications and toxicities. Arabian Journal of Chemistry 12(7) (2021) 908-931.

DOI: 10.1016/j.arabjc.2017.05.011

[2] M. Khatami, R.S. Varma, N. Zafarnia, H. Yaghoobi, M. Sarani, V.G. Kumar, Applications of green synthesized Ag, ZnO and Ag/ZnO nanoparticles for making clinical antimicrobial wound-healing bandages. Sustainable Chemistry and Pharmacy 10 (2018) 9–15.

DOI: 10.1016/j.scp.2018.08.001

[3] V. Tiwari, N. Mishra, K. Gadani, P.S. Solanki, N.A. Shah, M. Tiwari, Mechanism of anti-bacterial activity of Zinc oxide nanoparticle against carbapenem-resistant Acinetobacter baumannii. Frontiers in Microbiology 1218(9) (2018) 1-10.

DOI: 10.3389/fmicb.2018.01218

[4] M. Ebadi, M.R. Zolfaghari, S.S. Aghaei, M. Zargar, M. Shafiei, H.S. Zahiric, K.A. Noghabi, A bio-inspired strategy for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the cell extract of cyanobacterium Nostoc sp. EA03: from biological function to toxicity evaluation. RSC Advances 9 (2019) 23508–23525.

DOI: 10.1039/c9ra03962g

[5] L. Castillo-Henríquez, K. Alfaro-Aguilar, J. Ugalde-Álvarez, L. Vega-Fernández, G. Montes de Oca-Vásquez, J.R. Vega-Baudrit, Green synthesis of gold and silver nanoparticles from plant extracts and their possible applications as antimicrobial agents in the agricultural area. Nanomaterials 10 (2020) 1763.

DOI: 10.3390/nano10091763

[6] J.O. Primo, C. Bittencourt, S. Acosta, A. Sierra-Castillo, J.F. Colomer, S. Jaerger, V.C. Teixeira, F.J. Anaissi, Synthesis of zinc oxide nanoparticles by ecofriendly routes: Adsorbent for copper removal from wastewater. Frontiers in Chemistry 8 (2020) 571790.

DOI: 10.3389/fchem.2020.571790

[7] T.U.D. Thi, T.T. Nguyen, Y.D. Thi, K.H.T. Thi, B.T. Phan, K.N. Pham, Green synthesis of ZnO nanoparticles using orange fruit peel extract for antibacterial activities. RSC Advances 10 (2020) 23899–23907.

DOI: 10.1039/d0ra04926c

[8] N.A. Al-Dhabi, M.V. Arasu, Environmentally friendly green approach for the production of zinc oxide nanoparticles and their anti-fungal, ovicidal, and larvicidal properties. Nanomaterials 8 (2018) 500.

DOI: 10.3390/nano8070500

[9] R.K. Shah, F. Boruah, N. Parween, Synthesis and characterization of ZnO nanoparticles using leaf extract of Camelia sinensis and evaluation of their antimicrobial efficacy. International Journal of Current Microbiology and Applied Sciences 4 (2019) 444–450.

[10] M. Sánchez, E. González-Burgos, I. Iglesias, M.P. Gómez-Serranillos, Pharmacological update properties of aloe vera and its major active constituents. Molecules 25(6) (2020) 1324.

DOI: 10.3390/molecules25061324

[11] E.H. Kaparakou, C.D. Kanakis, M. Gerogianni, M. Maniati, K. Vekrellis, E. Skotti, P.A. Tarantilis, Quantitative determination of aloin, antioxidant activity, and toxicity of Aloe vera leaf gel products from Greece. Journal of the Science of Food and Agriculture 101(2) (2021) 414-423.

DOI: 10.1002/jsfa.10650

[12] M.A. Abdul Talib, N.A. Mohd Said, N.F. Nor Azeme, M.S. Ghazali, N.A. Masdor, F. Salam, K. Ramachandran, Specificity of PCR primers for identification of Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola with ten plant bacterial pathogens. Transactions of the Malaysian Society of Plant Physiology 25(1) (2017) 244-246.

DOI: 10.3390/pathogens9030187

[13] W. Lu, L. Pan, H. Zhao, Y. Jia, Y. Wang, X. Yu, X. Wang, Molecular detection of Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola and Burkholderia glumae in infected rice seeds and leaves. The Crop Journal 2(6) (2014) 398 – 406.

DOI: 10.1016/j.cj.2014.06.005

[14] G. Marslin, K. Siram, Q. Maqbool, R.K. Selvakesavan, D. Kruszka, P. Kachlicki, G. Franklin, Secondary metabolites in the green synthesis of metallic nanoparticles. Materials 11(6) (2018) 940.

DOI: 10.3390/ma11060940

[15] A. Aldalbahi, S. Alterary, R.A.A. Almoghim, M.A. Awad, N.S. Aldosari, S.F. Alghannam, A.N. Alabdan, S. Alharbi, B.A. Mohammed Alateeq, A.A. Al Mohsen, M.A. Alkathiri, R.A. Alrashed, Greener synthesis of zinc oxide nanoparticles: Characterization and multifaceted applications. Molecules 25 (2020) 4198-4212.

DOI: 10.3390/molecules25184198

[16] Y.W. He, X.Q. Cao, A.R. Poplawsky, Chemical structure, biological roles, biosynthesis and regulation of yellow xanthomonadin pigments in the phytopathogenic genus Xanthomonas. Molecular Plant-Microbe Interactions 33(5) (2020) 705-714.

DOI: 10.1094/mpmi-11-19-0326-cr

[17] K.M. Reddy, K. Feris, J. Bell, D.G. Wingett, C. Hanley, A. Punnoose, Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems. Applied Physics Letters 90(21) (2007) 213902.

DOI: 10.1063/1.2742324

[18] Y.N. Slavin, J. Asnis, U.O. Häfeli, H. Bach, Metal nanoparticles: Understanding the mechanisms behind antibacterial activity. Journal of Nanobiotechnology 15 (2017) 65.

DOI: 10.1186/s12951-017-0308-z

[19] K.R. Raghupathi, R.T. Koodali, A.C. Manna, Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir 27 (2011) 4020–4028.

DOI: 10.1021/la104825u

[20] Q.U.A. Naqvi, A. Kanwal, S. Qaseem, M. Naeem, S.R. Ali, M. Shaffique, M. Maqbool, Size-dependent inhibition of bacterial growth by chemically engineered spherical ZnO nanoparticles. Journal of Biological Physics 45 (2019) 147–159.

DOI: 10.1007/s10867-019-9520-4

[21] I. Kahramanoğlu, C.Y. Chen, J.Y. Chen, C.P. Wan, Chemical constituents, antimicrobial activity and food preservative characteristics of Aloe vera gel. Agronomy 9 (2019) 831.

DOI: 10.3390/agronomy9120831

[22] M.K. Hayat, T. Kumar, N. Ahmad, S.A. Jan, W. Sajjad, S. Faisal, B.X. Gao, K. Khan, Z.U. Rahman, B.H. Abbas, In vitro antimicrobial activity of Aloe vera L. extracts against pathogenic bacteria and fungi. Mycopath 14(1) (2016) 21-27.

[23] V.A. Ferro, F. Bradbury, P. Cameron, E. Shakir, S.R. Rahman, W.H. Stimson, In vitro susceptibilities of Shigella flexneri and Streptococcus pyogenes to inner gel of Aloe barbadensis Miller. Antimicrobial Agents and Chemotherapy 47(3) (2003) 1137-1139.

DOI: 10.1128/aac.47.3.1137-1139.2003

[24] X.L. Chen, S.F. Wei, Q. Yan, F.K. Huang, Z.F. Ma, R.F. Li, Z.L. Chen, W.H. Yan, K.H. Li, Virulence and DNA fingerprinting analysis of Xanthomonas oryzae pv. oryzae identify a new pathotype in Guangxi, South China. Journal of Basic Microbiology 59(11) (2019) 1082-1091.

DOI: 10.1002/jobm.201900354

[25] A. Saad, H. Habibuddin, Pathotypes and virulence of Xanthomonas oryzae causing bacterial blight disease of rice in Peninsular Malaysia. Journal of Tropical Agriculture and Food Science 38(2) (2010) 257– 266.