Prolonged Release and Cytocompatibility on Immortalized Keratinocytes of CoQ10-Loaded Nanostructured Lipid Carrier

Abstract:

The objective was to manufacture a nanostructured lipid carrier (NLC) for Coenzyme Q10, and to investigate its prolonged release and cytocompatibility of CoQ10-NLC incubated with HaCaT cells. CoQ10-NLC was prepared by hot high-pressure homogenization technique. The characterization of the CoQ10-NLC was determined by size analysis, polydispersity index (PDI), zeta potential assay, in vitro release and cytocompatibility. To analyze the cytocompatibility of CoQ10-NLC, cell viability was investigated by MTT measurement. Morphology of cells was evaluated by HE staining. Cells were exposed to CoQ10-NLC and nuclear morphology were determined using Hoechst 33342 staining. Time-lapse imaging was used to illustrate the dynamics of cell movements. Release investigation exhibited a prolonged release of CoQ10-NLC. MTT measurement, HE and Hoechst 33342 staining corroborated that CoQ10-NLC possessed good cytocompatibility on HaCaT cells. Observation with time-lapse images further confirmed that CoQ10-NLC showed good cytocompatibility. The results demonstrated that CoQ10-NLC with prolonged release had good cytocompatibility.

• Periodical: Journal of Nano Research (Volume 30)
• Pages: 128-141
• DOI: https://doi.org/10.4028/www.scientific.net/JNanoR.30.128
• Online since: March 2015
• Authors: Jian Min Wang, Qiang Xia*
• Keywords: Coenzyme Q10 (CoQ10), Cytocompatibility, HaCaT Cells, Nanostructured Lipid Carrier (NLC), Prolonged Release, Time-Lapse Imaging

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