Mesoporous carbon (MCs) was synthesized using mesoporous silica as a template. Prepared mesoporous carbon materials were found to have high surface area and large pore volume with uniform pore size. Resulting materials showed a different pore arrangement from mesoporous silica templates. Pt catalysts supported on mesoporous carbon prepared were applied to an anode catalyst for Direct Methanol Fuel Cell (DMFC). Results indicate that a mesoporous carbon having 3-dimensional pore system (3D-MC) had higher catalytic activity than 2-dimensional one (2D-MC) due to a favorable pore structure in methanol electro-oxidation.