Genotoxicity Effects of Ceramic Coatings Applied on Metallic Substrates Using Single Cell Gel Electrophoresis Assay In Vitro

Abstract:

Sol-Gel coatings are a good choice for protection and bioactivation of metals used as dentistry and standard surgical implant materials. These films should both prevent degradation of the substrates by wear or corrosion, and bioactivate the material for inducing the formation of a hydroxyapatite (HA) rich layer onto the material surface, thereby permitting a natural bonding to living tissues. The aim of this work was to estimate the clastogenicity in vitro by Single Cell Gel lectrophoresis Assay (SCGE) or “comet” assay of coatings of TiN applied by magnetron sputtering and of hybrid layers obtained by Sol-Gel containing glass, glass-ceramic and HA particles on stainless steel AISI 304. Six test specimens were prepared: AISI 304 Stainless Steel coated with an hybrid silica single film (SF), applied by sol-gel process, AISI 304 SS coated with double film with bioactive glass (DFG), glass-ceramics (DFGC) and HA (DFHA) particles, AISI 304 SS coated with TiN multi films (MFTiN) applied by PVD and bare AISI 304 SS (304SS). Significantly lower DNA migration (p>0.005) was observed in the cells of the cultures corresponding to the samples coated with SF, DFG, DFGC, DFHA and MFTiN respect to the bare 304 SS. The comparison between negative control and the same coated samples did not reveal any statistically significant difference (p>0.005) in clastogenicity in vitro evaluated by SCGE.

• Periodical: Key Engineering Materials (Volumes 284-286)
• Pages: 593-596
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.284-286.593
• Online since: April 2005
• Authors: Alejandro Peláez, Claudia P. García, A. Pareja, M.E. Márquez, Alejandro Toro, R. Castañeda, Pablo Abad
• Keywords: Coating, Genotoxicity, PVD, SCGE, Sol-Gel (SG)

References

[1] Gurappa, I., Surf. Coat Technol., Vol 161 (2002) p.70.

[2] Kannan, S., A. Balamurugan, and S. Rajeswari. Mat lett, Vol 57 (2003) p.2382.

[3] Innocenzi, P.C., et al. J. of Eur. Cer. Soc., Vol 10 (1992) p.431.

[4] Sanctis, O. d., et al. J. of Non Cryst. Solids, Vol 121 (1990) p.338.

[5] Galliano, P., et al.J. of Sol-Gel Sci. and Technology, Vol 13 (1998) p.723.

[6] Gallardo, J., et al. J of Sol-Gel Sci. and Technology, Vol 19 (2000) p.107.

[7] Gallardo, J., P. Galliano, and A. Durán. J of Sol-Gel Sci. and Technology, Vol 21 (2001) p.65.

[8] García, C., P. Galliano, and S. Cere. Mat. Lett., Vol 57 (2003) p.1810.

[9] Durán, A., et al. J of Mat Chem., Vol 14-14 (2004) p.2282.

[10] Ballarre, J., et al. J of Non Cryst. Solids, Vol 304 (2002) p.278.

[11] Singh NP. Mutat Res., Vol. 455 (2000) p.111.

[12] Konca K, Lankoff A, Banasik A, Lisowska H, Kuszewski T, Gozdz S, Koza Z, Wojcik A. Mutat Res., Vol. 534(1-2) (2003) p.15.

[13] Harris GK, Shi X. Mutat Res., Vol. 533(1-2) (2003) p.183.

[14] Christoffersen MR, Thyregod HC, Christoffersen J. Calcif Tissue Int., Vol 41-1 (1987) p.27.