Comparison of Validity between WST-1 and MTT Test in Bioceramic Materials

Abstract:

Cytotoxicity test was essential for the pre-clinical evaluation of bioceramics. Proliferation assays such as MTT, XTT and WST-1 were commonly used for measuring biocompatibility. WST-1 was more convenient than MTT because of its water-solubility and storage condition. The calcium phosphate glass and β-TCP have been used for bone substitute, and some magnetic ferrites have been used for hyperthermic treatment. L929, mouse fibroblast cell, was the representative cell-line for in vitro biocompatibility test. The extracts of test samples were prepared by ISO10993-12:2002. The biocompatibilities of the extracts were measured by MTT and WST-1 assay and their pH were measured with pH meter. The cellular survival rate of CPG was the lowest and the results of the WST-1 test showed results similar to those of the MTT test. Thus, proliferation assays using WST-1 may be conveniently and routinely applicable to pre-clinical evaluation of bioceramics.

• Periodical: Key Engineering Materials (Volumes 284-286)
• Pages: 585-588
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.284-286.585
• Online since: April 2005
• Authors: Kwang Mahn Kim, Sang Bae Lee, Se Ho Lee, Yong Keun Lee, Kyoung Nam Kim
• Keywords: Bioceramic, Biocompatibility, Cytotoxicity, MTT, Proliferation Assay, WST-1

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