Effect of Calcium Phosphate Addition to Alginate Microspheres: Modulation of Enzyme Release Kinetics and Improvement of Cell Adhesion

C.C. Barrias; C.C. Ribeiro; Daniel Rodrigues; M.C. Sá Miranda; Mario A. Barbosa

doi:10.4028/www.scientific.net/KEM.284-286.689

Paper Titles

Attachment of Blood Cells onto ZrO₂ and SiO₂-Containing Glass
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Behavior of Osteoblasts Cultured on Collagen Coated Bioactive Glass
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In Vitro Mesenchymal Stem Cell Culture Using Calcium Phosphate Glass Scaffold
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Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells
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Effect of Calcium Phosphate Addition to Alginate Microspheres: Modulation of Enzyme Release Kinetics and Improvement of Cell Adhesion
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Tensile and Compressive Behaviours and Properties of a Bone Analogue Biomaterial
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Competing Damage Modes in All-Ceramic Crowns: Fatigue and Lifetime
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Carboxymethylchitosan/Calcium Phosphate Hybrid Materials Prepared by an Innovative Auto-Catalytic Co-Precipitation Method
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Multi-Scale Structure of the Pinctada Mother of Pearl: Demonstration of a Continuous and Oriented Organic Framework in a Natural Ceramic
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Effect of Calcium Phosphate Addition to Alginate Microspheres: Modulation of Enzyme Release Kinetics and Improvement of Cell Adhesion

Abstract:

In this study, the addition of calcium phosphate powders to an alginate matrix was evaluated as a strategy to modulate enzyme release-kinetics from alginate microspheres and, simultaneously, to improve cell adhesion to the polymer. Pre-adsorption of the enzyme to the ceramic powders resulted in a more adequate release pattern. The ratio of ceramic-to-polymer had a pronounced effect on osteoblast adhesion to microspheres. Cells were only able to spread on microspheres with the highest percentage of ceramic (0.4 w/w using a 1.5% w/v alginate solution).