Effect of Calcium Phosphate Addition to Alginate Microspheres: Modulation of Enzyme Release Kinetics and Improvement of Cell Adhesion
In this study, the addition of calcium phosphate powders to an alginate matrix was evaluated as a strategy to modulate enzyme release-kinetics from alginate microspheres and, simultaneously, to improve cell adhesion to the polymer. Pre-adsorption of the enzyme to the ceramic powders resulted in a more adequate release pattern. The ratio of ceramic-to-polymer had a pronounced effect on osteoblast adhesion to microspheres. Cells were only able to spread on microspheres with the highest percentage of ceramic (0.4 w/w using a 1.5% w/v alginate solution).
Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.
C.C. Barrias et al., "Effect of Calcium Phosphate Addition to Alginate Microspheres: Modulation of Enzyme Release Kinetics and Improvement of Cell Adhesion ", Key Engineering Materials, Vols. 284-286, pp. 689-692, 2005