Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells
This paper demonstrated a simple technique to seed the hydroxyapatite (HA) ceramic with 2-D cultured cells to establish an advanced mode of HA grafting. The HA beads with a through-hole were fabricated to have an oblate shape to keep an opening of the through-hole contact with 2-D cultured cells layer. The seeding was performed by leaving the HA beads in a culture dish with MG63 cells in culture. The through-hole trapped MG63 cells in a sheet form by 1-day seeding. After 5 days, the number of trapped cells increased about nine fold, forming cell clumps in the through-hole. The seeding of the HA beads was considered an easy and practical method to provide HA/Cell hybrids that would realize active bone forming orthopedic treatment.
Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.
K. Teraoka et al., "Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells", Key Engineering Materials, Vols. 284-286, pp. 683-688, 2005