Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells

Abstract:

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This paper demonstrated a simple technique to seed the hydroxyapatite (HA) ceramic with 2-D cultured cells to establish an advanced mode of HA grafting. The HA beads with a through-hole were fabricated to have an oblate shape to keep an opening of the through-hole contact with 2-D cultured cells layer. The seeding was performed by leaving the HA beads in a culture dish with MG63 cells in culture. The through-hole trapped MG63 cells in a sheet form by 1-day seeding. After 5 days, the number of trapped cells increased about nine fold, forming cell clumps in the through-hole. The seeding of the HA beads was considered an easy and practical method to provide HA/Cell hybrids that would realize active bone forming orthopedic treatment.

Info:

Periodical:

Key Engineering Materials (Volumes 284-286)

Main Theme:

Edited by:

Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.

Pages:

683-688

DOI:

10.4028/www.scientific.net/KEM.284-286.683

Citation:

K. Teraoka et al., "Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells", Key Engineering Materials, Vols. 284-286, pp. 683-688, 2005

Online since:

April 2005

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Price:

$35.00

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