Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells

Kay Teraoka; Takao Saito; Yoshiyuki Yokogawa

doi:10.4028/www.scientific.net/KEM.284-286.683

Paper Titles

Osteogenic Effect of Genistein on In Vitro Bone Formation by Human Bone Marrow Cell Culture - For Development of Advanced Bio-Artificial Bone
p.667

Attachment of Blood Cells onto ZrO₂ and SiO₂-Containing Glass
p.671

Behavior of Osteoblasts Cultured on Collagen Coated Bioactive Glass
p.675

In Vitro Mesenchymal Stem Cell Culture Using Calcium Phosphate Glass Scaffold
p.679

Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells
p.683

Effect of Calcium Phosphate Addition to Alginate Microspheres: Modulation of Enzyme Release Kinetics and Improvement of Cell Adhesion
p.689

Tensile and Compressive Behaviours and Properties of a Bone Analogue Biomaterial
p.693

Competing Damage Modes in All-Ceramic Crowns: Fatigue and Lifetime
p.697

Carboxymethylchitosan/Calcium Phosphate Hybrid Materials Prepared by an Innovative Auto-Catalytic Co-Precipitation Method
p.701

HomeKey Engineering MaterialsKey Engineering Materials Vols. 284-286Preparation of Hydroxyapatite Ceramic Unit with a...

Preparation of Hydroxyapatite Ceramic Unit with a Trap for 2-D Cultured Cells

Abstract:

This paper demonstrated a simple technique to seed the hydroxyapatite (HA) ceramic with 2-D cultured cells to establish an advanced mode of HA grafting. The HA beads with a through-hole were fabricated to have an oblate shape to keep an opening of the through-hole contact with 2-D cultured cells layer. The seeding was performed by leaving the HA beads in a culture dish with MG63 cells in culture. The through-hole trapped MG63 cells in a sheet form by 1-day seeding. After 5 days, the number of trapped cells increased about nine fold, forming cell clumps in the through-hole. The seeding of the HA beads was considered an easy and practical method to provide HA/Cell hybrids that would realize active bone forming orthopedic treatment.