A Potent Osteogenic Composite of Stem Cells – Tricalcium Phosphate Ceramic Enhanced the Posterior Spinal Fusion

Abstract:

Bone marrow mesenchymal stem cells (BMSCs) possess a high replicative capacity and have the capacity to differentiate into various connective tissue cell types. With the advance in cell culture technique, the BMSCs have been induced to differentiate to osteoblastics linage. To improve the situation of non-union in posterior spinal fusion (PSF), tissue engineering approach to combine BMSCs supported by the calcium phosphate ceramics was applied in PSF and its effect was investigated in the present study. Autologous BMSCs from 16-week-old rabbit tibiae were expanded and induced to differentiate into osteoblastic cells with defined medium and osteogenic supplement. Calcium phosphate ceramic (CPC) was used as the scaffold to deliver the cells to the standardized rabbit posterior spinal fusion model. The assessment of bone mineral and fusion mass volume was conducted at week 7 post-operation with quantitative computed tomography and micro-computed tomography. When compared with control, the composite of BMSCs with CPC enhanced the fusion mass volume by 40% (p<0.05) and bone mineral content in the CPC was 7% (p=0.05) higher. Our study showed that additional BMSCs at the fusion site of PSF did provide extra resource for new bone formation and enhanced the fusion rate.

• Periodical: Key Engineering Materials (Volumes 288-289)
• Pages: 39-42
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.288-289.39
• Online since: June 2005
• Authors: H.Y. Yeung, J.Z. Liu, Ling Qin, Yun Yu Hu, Chang Wei Lu, K.M. Lee, Y.M. Chiu, Jack C.Y. Cheng
• Keywords: Bone Marrow Stem Cells, Calcium Phosphate Ceramic, Osteogenesis, Posterior Spinal Fusion

References

[1] Bruder SP, Fox BS: Tissue engineering of bone. Cell based strategies. Clin OrthopS68-S83, (1999).

[2] Schimandle JH, Boden SD: Spinal Fusion: Bone Grafts and bone Graft Substitutes for Spinal Fusion. In: The Spine, Rothman-Simeone pp.1610-1629. Ed by HN Herkowitz, RH Rothman, and FA Simeone. Philadephia, W.B. Saunders, 1999 A B C D.

DOI: 10.1016/b978-1-4160-6726-9.00067-5

[3] Wang W, Hu Y, Lu S: Study on the bone marrow mesenchymal stem cells differentiate into chondrocyte in vitro. Chin J Surg 38: 160, (2000).

[4] Lennon DP, Haynesworth SE, Young RG, Dennis JE, Caplan AI: A chemically defined medium supports in vitro proliferation and maintains the osteochondral potential of rat marrow-derived mesenchymal stem cells. Exp Cell Res 219: 211-222, (1995).

DOI: 10.1006/excr.1995.1221

[5] Uchida A, Kikuchi T, Shimomura Y: Osteogenic capacity of cultured human periosteal cells. Acta Orthop Scand 59: 29-33, (1988).

DOI: 10.3109/17453678809149339

[6] Cui C, Hu Y, Lei W: The influence of injectable osteoinductive material with rhBMP-2 and bFGF on the proliferation and ultrastructure of marrow stromal cell on rabit. Chin J Clin Rehabilitation 6: 2376-2377, (2002).

[7] Dou Y, Shun L, Hu Y: Construction and culture in vitro of deantigen bone xenograft block loaded with MSC and alginate. Modern Rehabilitation 5: 41-43, (2001).

[8] Spivak JM, Hasharoni A: Use of hydroxyapatite in spine surgery. Eur Spine J 10 Suppl 2: S197-S204, (2001).

DOI: 10.1007/s005860100286

[9] Martin I, Muraglia A, Campanile G, Cancedda R, Quarto R: Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. Endocrinology 138: 4456-4462, (1997).

DOI: 10.1210/endo.138.10.5425

[10] Yaylaoglu MB, Yildiz C, Korkusuz F, Hasirci V: A novel osteochondral implant. Biomaterials 20: 1513-1520, (1999).

DOI: 10.1016/s0142-9612(99)00062-9

[11] Cheng JC: Current concepts of surgical treatment of adolescent idiopathic scoliosis. In: Recent Advances in Orthopaedics Ed by S Bhan. India, Jaypee Brothers, (1994).

[12] DePalma AF, Rothman RH: The nature of pseudarthrosis. Clinical Orthopaedics & Related Research 59: 113-118, (1968).

DOI: 10.1097/00003086-196807000-00007

[13] Steinmann JC, Herkowitz HN: Pseudarthrosis of the spine. Clinical Orthopaedics & Related Research80-90, (1992).

DOI: 10.1097/00003086-199211000-00011

[14] Cheng JC, Guo X, Law LP, Lee KM, Chow DH, Rosier R: How does recombinant human bone morphogenetic protein-4 enhance posterior spinal fusion? Spine 27: 467-474, (2002).

DOI: 10.1097/00007632-200203010-00006

[15] Guo X, Lee KM, Law LP, Chow HK, Rosier R, Cheng CY: Recombinant human bone morphogenetic protein-4 (rhBMP-4) enhanced posterior spinal fusion without decortication. J Orthop Res 20: 740-746, (2002).

DOI: 10.1016/s0736-0266(01)00167-x

[16] Cinotti G, Patti AM, Vulcano A, Della RC, Polveroni G, Giannicola G, Postacchini F: Experimental posterolateral spinal fusion with porous ceramics and mesenchymal stem cells. J Bone Joint Surg Br 86: 135-142, (2004).

DOI: 10.1302/0301-620x.86b1.14308

[17] Boo JS, Yamada Y, Okazaki Y, Hibino Y, Okada K, Hata K, Yoshikawa T, Sugiura Y, Ueda M: Tissue-engineered bone using mesenchymal stem cells and a biodegradable scaffold. J Craniofac Surg 13: 231-239, (2002).

DOI: 10.1097/00001665-200203000-00009

[18] Kadiyala S, Young RG, Thiede MA, Bruder SP: Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro. Cell Transplantation 6: 125-134, (1997).

DOI: 10.1177/096368979700600206

[19] Bruder SP, Fink DJ, Caplan AI: Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem 56: 283-294, (1994).

DOI: 10.1002/jcb.240560303