Hydroxyapatite/Chondroitin Sulfate Microparticles with Different Particle Size and its Adsorption Ability of Proteins
Hydroxyapatite/chondroitin sulfate (HAp/ChS) microparticles with the perfectly spherical shape and the averaged particle sizes of 4.1, 7.8, 19.9 and 29.4 µm were fabricated by a spray dry method under the different atomizing pressures and the concentrations of suspensions. The contents of ChS in the microparticles were varied at 1.49, 3.18, 7.82, 14.2 wt%, and the ChS elution rate in distilled water from the microparticles was increased with the increase of ChS contents. The adsorption isotherms of cytochrome C on the microparticles in 1/10 diluted phosphate buffer saline (PBS) were followed to the Langmuir’s equation regardless of the change of ChS contents, while those of catalase were not followed. The HAp/ChS microparticles can adsorb greater amount of cytochrome C than pure HAp microparticles, but less amount of catalase than pure HAp. The electrostatic interaction between the proteins and ChS was of great importance in the adsorption properties.
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
H. Watanabe et al., "Hydroxyapatite/Chondroitin Sulfate Microparticles with Different Particle Size and its Adsorption Ability of Proteins", Key Engineering Materials, Vols. 309-311, pp. 533-536, 2006