Expression of Bioactivity and Biocompatibility Testing of Bioactive Glass Modified Zinc Phosphate Luting Cement
The bioactivity and biocompatibility of a zinc phosphate luting cement (HARVARD, Richter & Hoffmann, Dental-GmbH, Berlin) which was modified by Bioglass® (PerioGlas® Synthetic Bone Graft Particulate, US Biomaterials), was evaluated in vitro with human lung fibroblasts (MRC-5), baby hamster kidney fibroblasts (BHK) and rat pulp cells (RPC) by XTT and BrdU assays. A thin Ca-P layer was grown on the surface of Bioglass®-modified zinc phosphate cement specimens after immersion in SBF for 7 days and remained constant after 16 days immersion time. The incorporation of Bioglass® powder in zinc phosphate specimens resulted in equal or increased cell attachment and activity for almost all cell lines examined without any apparent impact on mechanical or physicochemical properties of the cement, although this needs further documentation. The combination of these two methods in determining the biocompatibility of Bioglass®-modified zinc phosphate cements showed that cells not only attached well on modified specimens but were actively synthesizing DNA after 72h of incubation.
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
E. Kontonasaki et al., "Expression of Bioactivity and Biocompatibility Testing of Bioactive Glass Modified Zinc Phosphate Luting Cement ", Key Engineering Materials, Vols. 309-311, pp. 813-816, 2006