Hydroxyapatite/Chondoroitin Sulfate Microparticles as Time-Controlled Release Carrier of Proteins

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Time-controlled releases of proteins from hydroxyapatite/chondroitin sulfate (HAp/ChS) spherical microparticles were achieved by the addition of zinc cation into the mixture solutions of HAp/ChS and protein as a novel formulation. The initial bursts of proteins, such as cytochrome c and bovine serum albumin, were apparently suppressed by the amount of zinc cation, which could be attributed to the formation of coordinate bonds of zinc cation among proteins and/or ChS moleculars. The increase of molecular lengths of ChS chains decreased the adsorbed amount of proteins, which did not apparently affected to the release of proteins.

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Periodical:

Key Engineering Materials (Volumes 330-332)

Main Theme:

Edited by:

Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu

Pages:

1009-1012

Citation:

H. Watanabe et al., "Hydroxyapatite/Chondoroitin Sulfate Microparticles as Time-Controlled Release Carrier of Proteins", Key Engineering Materials, Vols. 330-332, pp. 1009-1012, 2007

Online since:

February 2007

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$38.00

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[10] A. Ito et al. Mater. Sci. & Eng. C 22 (2002), pp.21-25 Fig. 3 Release patterns of the model proteins from the microparticles formulated with zinc cation; (a) Hb, (b) cyt c and (c) LYZ (n=4). (b) (a) (c).

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[2] 2. 5 0 20 40 60 80 100 120 140 160 180.

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[2] 2. 5 0 20 40 60 80 100 120 140 160 180.

[2] [4] [6] [8] [10] [12] [14] [16] 0 20 40 60 80 100 120 140 160 180.

[2] [4] [6] [8] [10] [12] [14] [16] 0 20 40 60 80 100 120 140 160 180.

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[40] [50] [60] [70] [80] [90] 100 0 20 40 60 80 100 120 140 160 180 Soaking time (hour) Soaking time (hour) Soaking time (hour) Rate of released protein (%) Rate of released protein (%) Rate of released protein (%) HAp+3Zn HAp/ChS(15kDa)2%+3Zn HAp/ChS(20kDa)2%+3Zn HAp/ChS(35kDa)2%+3Zn HAp+3Zn HAp/ChS(15kDa)2%+3Zn HAp/ChS(20kDa)2%+3Zn HAp/ChS(35kDa)2%+3Zn HAp+3Zn HAp/ChS(15kDa)2%+3Zn HAp/ChS(20kDa)2%+3Zn HAp/ChS(35kDa)2%+3Zn HAp+3Zn HAp/ChS(15kDa)2%+3Zn HAp/ChS(20kDa)2%+3Zn HAp/ChS(35kDa)2%+3Zn.

DOI: https://doi.org/10.1515/9783110317534.67

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