Effect of Hydroxyapatite-Tricalcium Phosphate Micro-Particles on the Cellular Interaction of In Situ Hyaluronic Acid-Poly(Ethylene Oxide) Hydrogel

Abstract:

In vitro cell behaviors of calvarial osteoblasts (MC3T3) were evaluated by seeding them on both the surface and inside of in situ hyaluronic acid-poly(ethylene oxide) (HA-PEO) hydrogel, either after or before incorporation of mixture micro-particles of hydroxyapatite-β-tricalcium phosphate on/inside the hydrogel, respectively. Cellular behaviors such as adhesion and proliferation on the surface and inside the gel were evaluated with light microscopy and a microplate reader by focusing on the interactions of cell-HA-PEO as well as cell-hydroxyapatitetricalcium phosphate micro-particle surface in the gel. Cell adhesion and spreading seemed to be enhanced by supplying the micro-particles to the inside the HA-PEO hydrogel, compared to the results of the HA-PEO hydrogel itself.

• Periodical: Key Engineering Materials (Volumes 342-343)
• Pages: 765-768
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.342-343.765
• Online since: July 2007
• Authors: Mi Sook Kim, Yoon Jeong Choi, Gun Woo Kim, In Sup Noh, Yong Doo Park, Kyu Back Lee, In Sook Kim, Soon Jung Hwang
• Keywords: Bone Cell Interaction, Hyaluronic Acid (HA), Hydroxyapatite (HAP), Poly(ethylene oxide) (PEO), Tricalcium Phosphate

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