Observation of Proliferation and Attachment of Three Different Cell Types on Nano-Fibrous Silk Film & Scaffold

Hyun Sook Baek; Young Hwan Park; Ki Chang Seok; Jong Chul Park; Don Kyun Rah

doi:10.4028/www.scientific.net/KEM.342-343.85

Paper Titles

Preparation and Characterization of Small Intestinal Submucosa Coated with Alginate/Gelatin as a Wound Dressing
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The Biocompatibility of Silk Scaffold for Tissue Engineered Ligaments
p.73

Biodegradable Porous PCL/HA Scaffolds for Bone Tissue Engineering
p.77

Toxicity Test of Wound Covering Material on Animals
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Observation of Proliferation and Attachment of Three Different Cell Types on Nano-Fibrous Silk Film & Scaffold
p.85

Human Cartilage Tissue Engineering with Pluronic and Cultured Chondrocyte Sheet
p.89

Growth of Smooth Muscle Cell and Endothelial Cell on PLGA Film Containing EGCG
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Effect of Three-Dimensional Scaffold Geometry on Chondrocyte Adhesion
p.97

Human Adipose Tissue-Derived Stem Cell Seeded Hyaluronic Acid-Collagen Scaffolds for Adipogenesis Investigated In Vivo
p.101

HomeKey Engineering MaterialsKey Engineering Materials Vols. 342-343Observation of Proliferation and Attachment of...

Observation of Proliferation and Attachment of Three Different Cell Types on Nano-Fibrous Silk Film & Scaffold

Abstract:

Attachment and viability of different cell types(fibrioblast, chondrocyte and osteoblast ) was observed on two forms of silk (mat & Three-dimensional scaffolds). The osteoblasts behaviors cultured on silk mat were significantly higher than that found on 3-D silk fibroin scaffold (3-D SF scaffold). In the MTT assay, the cell viability of fibroblasts, chondrocyte and osteoblasts seeded on 2-D nanofiber mat was (2-D mat) significantly higher than that found on 3-D SF scaffold. Similar result could be seen from SEM observation and cell attachment study. However, alkaline phosphatase activity was significantly increased on 3-D SF scaffold than on2-D nanofiber