Observation of Proliferation and Attachment of Three Different Cell Types on Nano-Fibrous Silk Film & Scaffold

Abstract:

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Attachment and viability of different cell types(fibrioblast, chondrocyte and osteoblast ) was observed on two forms of silk (mat & Three-dimensional scaffolds). The osteoblasts behaviors cultured on silk mat were significantly higher than that found on 3-D silk fibroin scaffold (3-D SF scaffold). In the MTT assay, the cell viability of fibroblasts, chondrocyte and osteoblasts seeded on 2-D nanofiber mat was (2-D mat) significantly higher than that found on 3-D SF scaffold. Similar result could be seen from SEM observation and cell attachment study. However, alkaline phosphatase activity was significantly increased on 3-D SF scaffold than on2-D nanofiber

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon

Pages:

85-88

DOI:

10.4028/www.scientific.net/KEM.342-343.85

Citation:

H. S. Baek et al., "Observation of Proliferation and Attachment of Three Different Cell Types on Nano-Fibrous Silk Film & Scaffold", Key Engineering Materials, Vols. 342-343, pp. 85-88, 2007

Online since:

July 2007

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Price:

$35.00

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