Reconstruction of Liver Organoid Using an Apatite-Fiber Scaffold, a Radial-Flow Bioreactor and FCL-4 Cells of Hepatocyte Model

Abstract:

We have previously developed apatite-fiber scaffolds (AFSs) for bone tissue engineering using single-crystal apatite fibers and carbon beads. In the present investigation, we examined the possibility of reconstruction of a liver organoid via three-dimensional (3D) culture of hepatocytes using the AFSs and the radial-flow bioreactor (RFB), aiming to apply the scaffold as a matrix for regeneration of a real organ. FLC-4 cells were used as a model of hepatocyte. The cells were well-viable in the RFB settled with AFSs over a period for 28 d, compared with the cases of cellulose beads and apatite beads with high porosity of 85%. We conclude that the present AFS may be a promising scaffold for tissue engineering of liver.

• Periodical: Key Engineering Materials (Volumes 361-363)
• Pages: 1165-1168
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.361-363.1165
• Online since: November 2007
• Authors: Mamoru Aizawa, A. Hiramoto, H. Maehashi, Tomokazu Matsuura
• Keywords: Apatite-Fiber Scaffold (AFS), Hydroxyapatite (HAP), Liver Organoid, Radial Flow Bioreactor, Tissue Engineering

References

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[6] M. Aizawa, A. E. Porter, S. M. Best, W. Bonfield: Biomaterials Vol. 26 (2005), p.3427. Glucose / g・・cm-3 Time / day.

7 21 28 1. 0 1. 5 2. 0 0. 5.

[14] ：：Medium change AFS2000 Cellulose beads Apatite beads Time / day Lactic acid / mmol・・cm-3.

[6] [12] [7] 21 28.

[14] [18] AFS2000 Cellulose beads Apatite beads (a) (b) Glucose / g・・cm-3 Time / day.

7 21 28 1. 0 1. 5 2. 0 0. 5.

[14] ：：Medium change AFS2000 Cellulose beads Apatite beads Glucose / g・・cm-3 Time / day.

7 21 28 1. 0 1. 5 2. 0 0. 5.

[14] ：：Medium change AFS2000 Cellulose beads Apatite beads AFS2000 Cellulose beads Apatite beads Time / day Lactic acid / mmol・・cm-3.

[6] [12] [7] 21 28.

[14] [18] AFS2000 Cellulose beads Apatite beads Time / day Lactic acid / mmol・・cm-3.

[6] [12] [7] 21 28.

[14] [18] AFS2000 Cellulose beads Apatite beads AFS2000 Cellulose beads Apatite beads AFS2000 Cellulose beads Apatite beads (a) (b) Figure 4 Changes of concentrations of (a) glucose and (b) lactic acid in the medium of RFB system. Figure 5 the microstructure of liver organoid tissue-engineered using the AFS2000, FLC-4 cells, and the RFB system.

DOI: 10.7554/elife.45239.004