Preparation and In Vitro Characterization of Wollastonite Doped Tricalcium Phosphate Bioceramics
In this work a new kind of CaSiO3-doped α-Ca3(PO4)2 ceramic materials, with compositions lying outside the field of the Ca3(PO4)2 solid solution in the system Ca3(PO4)2- CaSiO3, were obtained and some of their properties, relevant for bone repairing, were studied in vitro. Crystalline α-Ca3(PO4)2 solid solution and minor amounts of non-equilibrium residual glass were the only phases in the materials containing 2 and 5 wt% of CaSiO3. α-Ca3(PO4)2, crystalline eutectic-like phase and residual glass were observed for sample containing 15 and 20 wt% of CaSiO3. The mechanical strength improved for all the doped ceramics with regard to un-doped Ca3(PO4)2. The release of ionic Ca and Si in simulated physiological conditions increased with the content of CaSiO3 and favored α-Ca3(PO4)2 surface transformation. The soluble components extracted from the CaSiO3-doped α-Ca3(PO4)2 bioceramics were not cytotoxic to human fibroblastlike cells. Initial cell adhesion onto the surface of the materials seemed to be partially hindered by surface reactivity and remodeling, however those cells adhered to the experimental bioceramics were viable and proliferated normally.
Guy Daculsi and Pierre Layrolle
R. García Carrodeguas et al., "Preparation and In Vitro Characterization of Wollastonite Doped Tricalcium Phosphate Bioceramics", Key Engineering Materials, Vols. 361-363, pp. 237-240, 2008