Enhancement of the Mechanical Property of Apatite-Fiber Scaffold Using Type I-Collagen

Abstract:

We have successfully fabricated apatite-fiber scaffolds (AFSs) that enable three-dimensional cell culture. The AFSs possessing large pores of 100~250 μm and micro pores of about 5 μm were fabricated by firing the green compacts consisting of the single-crystal apatite fibers and the carbon beads with a size of 150 μm. In order to enhance the mechanical properties of the AFSs, we have improved the process of AFS fabrication: Collagen gel (type I) solutions were introduced into the pores in the scaffolds; in addition, the resulting apatite/collagen scaffolds were chemically modified by thermally dehydrated cross-linking. Actually, the results of compressive strength tests show that the value of the AFS with chemically cross-linked I-collagen was about twice as high as that of the conventional AFS without I-collagen. We conclude that combination of I-collagen and thermal dehydrated cross-linking is effective for enhancement of the mechanical properties of AFSs.

• Periodical: Key Engineering Materials (Volumes 361-363)
• Pages: 943-946
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.361-363.943
• Online since: November 2007
• Authors: S. Motojima, N. Igeta, Michiyo Honda, Nobuyuki Kanzawa, Mamoru Aizawa
• Keywords: Apatite Fibre, Hydroxyapatite (HAP), I-Collagen, Scaffold, Tissue Engineering

References

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DOI: 10.1097/01.brs.0000217679.46489.1b

02.

05.

1.

1.

1.

1.

1% Thermal dehydrated cross-linking 110 ºC 120 ºC 130 ºC 140 ºC Compressive strength [MPa].

[5] 2± 0. 3.

[5] 3± 0. 5.

[6] 4± 0. 5.

[7] 8± 0. 1.

[8] 9± 0. 3.

[10] 8± 0. 4.

[13] 1± 1. 3.

[11] 5± 0. 6 Specimens AFS1000(-) AFS1000(+0. 02) AFS1000(+0. 05) AFS1000(+0. 1) AFS1000(+0. 1)_110ºC AFS1000(+0. 1)_120ºC AFS1000(+0. 1)_130ºC AFS1000(+0. 1)_140ºC I-collagen.

02.

05.

1.

1.

1.

1.

1% Thermal dehydrated cross-linking 110 ºC 120 ºC 130 ºC 140 ºC Compressive strength [MPa].

[5] 2± 0. 3.

[5] 3± 0. 5.

[6] 4± 0. 5.

[7] 8± 0. 1.

[8] 9± 0. 3.

[10] 8± 0. 4.

[13] 1± 1. 3.

[11] 5± 0. 6.