Synthesis and Properties of Fluorescent Biological Molecules Labeled with Novel Silylated Perylene Derivative

Yuzuru Sato; Akiko Ozaki; Masahiro Hosaka; Tomohisa Moriguchi; Kazuo Shinozuka

doi:10.4028/www.scientific.net/KEM.459.63

Paper Titles

Solvent-Free Synthesis of Siloxanes and their Usage as Potential Cross-Coupling Reagents
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Preparation of Anisotropic Chitosan Hydrogel with Birefringence and Analysis of Gel Formation Process
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Thermosensitive Micelles Composed of Poly(lactide)-g-Poly(NIPAM-co-HEMA) Graft Copolymers
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Nano-Platelet Structure of Clay Materials Observed by Atomic Force Microscope
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Synthesis and Properties of Fluorescent Biological Molecules Labeled with Novel Silylated Perylene Derivative
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Effect of Organometallic Compounds on the Formation of Carbon Nanostructures by Pulsed Electric Discharge of Fluorine-Containing Organic Liquid between Metal Electrodes
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Control of Methanol Crossover Using a Perforated Metal Sheet for DMFC Application
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A Key Factor for the Actual Application of a Vapor Feed Passive DMFC Operated with High Concentration of Methanol
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Relationship between Dielectric Characteristic by DEP Levitation and Differentiation Activity for Stem Cells
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 459Synthesis and Properties of Fluorescent Biological...

Synthesis and Properties of Fluorescent Biological Molecules Labeled with Novel Silylated Perylene Derivative

Abstract:

We have synthesized novel perylene derivatives bearing a silyl function with modifiable functional groups as a feasible material for visualizing biological substances. The fluorescent perylene derivative was coupled to C-5 position of pyrimidine nucleotide and the nucleotide was subsequently incorporated to the middle position of oligoDNA using an automated DNA synthesizer. Also, the derivative was coupled with a cholesterol analog with different length of linking alkyl function. The resulting modified fluorescent oligoDNA exhibited a small change in its fluorescent signal upon hybridization with the complementary oligoDNA. While the cholesterol analogs bearing the silylated perylene exhibited marked fluorescent signal in living cells. The signal is stronger in the analog having longer linker function compared to the analog having shorter linker function. In each case, however, the signal was much prominent compared to that of dehydroergosterol (DHE), a fluorescent cholesterol analog widely used in biological study.

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Periodical:

Key Engineering Materials (Volume 459)

Pages:

63-66

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.459.63

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Online since:

December 2010

Authors:

Yuzuru Sato, Akiko Ozaki, Masahiro Hosaka, Tomohisa Moriguchi, Kazuo Shinozuka

Keywords:

Cholesterol, DNA, Fluorescent Probe, Perylene, Silylation

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