This paper presents a method to simulate the behavior of the cable harness using the absolute nodal coordinat formulation (ANCF) and to predict the fatigue life while computing the strain time history of the point of interest. Rigid body dynamics is applied for the robot system, while ANCF is used for the cable harness. The simulation is performed by using the dynamic analysis process. The material property of the cable is obtained by a test. A simplified model is prepared. With these data, the behavior of the cable is simulated and the fatigue life is predicted.