Hydroxyapatite Ceramics Including Bone Minerals Promote Differentiation of Osteoblasts Derived from Rat Bone Marrow Cells

Abstract:

Biological apatite presented in bone and teeth of mammals contains various minerals; thus, it has lots of defects with nano-scale sizes in the crystal structure. We fabricated hydroxyapatite ceramics including bone minerals (bone HAp ceramics) as model materials to clarify the relationship between nano-defect structure and bioactivity of biological apatite. The X-ray diffraction (XRD) results indicated that crystalline phase of pure and bone HAp ceramics were of HAp single phase. Chemical compositions of pure and bone HAp did not change before and after firing at 1000 °C for 5 h. Microstructure observed by high-resolution transmission electron microscope (HR-TEM) indicated that bone HAp ceramics contained more defects and strains compared to pure HAp ceramics. Osteoblasts derived from rat bone marrow cells (RBMC) were seeded on both pure and bone HAp ceramics. The level of differentiation into osteoblasts was examined by determining the content of alkaline phosphatase (ALP) for initial/middle stage and osteocalcin (OC) for late stage. The ALP activity normalized for DNA content of osteoblasts cultured on the bone HAp ceramics was higher than that of pure HAp ceramics. The OC amount normalized for DNA content of bone HAp ceramics was the almost same as that of pure HAp ceramics. These results demonstrate that the bone HAp ceramics may promote the differentiation into osteoblast.

• Periodical: Key Engineering Materials (Volumes 493-494)
• Pages: 320-324
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.493-494.320
• Online since: October 2011
• Authors: Mamoru Aizawa, Takuya Miki, Yumiko Yasutomi, Michiyo Honda, Hideyuki Yoshimura
• Keywords: Bone Minerals, Defect, Differentiation, Hydroxyapatite (HAP), Osteoblast, TEM

References

[1] H. Aoki, Medical Applications of Hydroxyapatite, Ishiyaku Euro America, Tokyo, 1994, p.174.

[2] S. Kannan, A. Rebelo, J.M.F. Ferreira, Novel synthesis and structural characterization of fluorine and chlorine co-substituted hydroxyapatites, J. Inorg. Biochem. 100 (2006) 1692–1697.

DOI: 10.1016/j.jinorgbio.2006.06.005

[3] G.W. Chinthaka Silva, Longzhou Ma, Oliver Hemmers, Dennis Lindle, Micro-structural characterization of precipitation-synthesized fluorapatite nano-material by transmission electron microscopy using different sample preparation techniques, Micron. 39 (2008).

DOI: 10.1016/j.micron.2007.09.004

[4] S. R. Kim, J. H. Lee, Y. T. Kim, D. H. Riu, S. J. Jung, Y. J. Lee, S. C. Chung, Y. H. Kim, Synthesis of Si, Mg substituted hydroxyapatites and their sintering behaviors, Biomater. 24 (2003) 1389-1398.

DOI: 10.1016/s0142-9612(02)00523-9

[5] X. L. Tang, X. F. Xiao, R. F. Liu, Structural characterization of silicon-substituted hydroxyapatite synthesized by a hydrothermal method, Mater. Lett. 59 (2005) 3841-3846.

DOI: 10.1016/j.matlet.2005.06.060

[6] M. Aizawa, N. Kanzawa, M. Matsumoto, Nano-structure of apatite-fiber scaffolds and its cell differentiation, J. Jpn. Soc. Biomater. 23 (2005) 336-342.

[7] T. Fujino, M. Aizawa, Preparation of apatite powder including bone minerals and its characterization, Arch. BioCeram. Res. 7 (2007) 175-178.

[8] C. Maniatopoulos, J. Sodek, A. H. Melcher, Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats, Cell Tissue Res., 254 (1988) 317-330.

DOI: 10.1007/bf00225804

[9] T. Miki, T. Fujino, Y. Yasutomi, T. Fukazawa, H. Yoshimura and M. Aizawa, Chemical composition and ultrastructure of apatite ceramics including bone minerals, Arch. BioCeram. Res. 10 (2010) 141-144.