Surface and Cell Affinity Properties of Chitosan-Gelatin-Hydroxyapatite Composite Films

Abstract:

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Addition of hydroxyapatite (HAp) into polymeric matrices and use them as supporting material for tissue engineering has gained attraction since hydroxyapatite (HAp) type crystals are the main inorganic components of the bone structure. In this study, composite films were prepared from chitosan (C) and gelatin (G) with addition of HAp, and the surface characterization and cell affinity properties were examined. The hydrophilicities and morphologies of the films were investigated by contact angle measurements and by scanning electron microscopy (SEM), respectively. Cell affinities were studied by using human osteosarcoma (SaOs-2) cell line. Crosslinked and HAp containing films were found to be more hydrophilic than that of the others, while surface free energy (SFE) values were almost similar for all films. Cell proliferation and spreading of SaOs-2 cells were higher for the films with high gelatin content and no HAp; whereas for HAp containing composites higher cell affinities were obtained for the samples which have high chitosan content.

Info:

Periodical:

Key Engineering Materials (Volumes 493-494)

Main Theme:

Edited by:

Eyup Sabri Kayali, Gultekin Goller and Ipek Akin

Pages:

337-342

DOI:

10.4028/www.scientific.net/KEM.493-494.337

Citation:

C. Isikli and N. Hasirci, "Surface and Cell Affinity Properties of Chitosan-Gelatin-Hydroxyapatite Composite Films", Key Engineering Materials, Vols. 493-494, pp. 337-342, 2012

Online since:

October 2011

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$35.00

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