Biocompatibility of Carbonated Hydroxyapatite Nanoparticles with Different Crystallinities

Elena Mavropoulos; Moema Hausen; Andrea Machado Costa; Silvia Raquel Albuquerque; Gutemberg Alves; J.M. Granjeiro; Alexandre Malta Rossi

doi:10.4028/www.scientific.net/KEM.493-494.331

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HomeKey Engineering MaterialsKey Engineering Materials Vols. 493-494Biocompatibility of Carbonated Hydroxyapatite...

Biocompatibility of Carbonated Hydroxyapatite Nanoparticles with Different Crystallinities

Abstract:

Carbonated apatite (CHA) is commonly considered a promising synthetic material for biomedical applications in orthopedic and dental surgery due to its biocompatibility, bioresorption and bioactivity. CHA5, CHA37 and CHA90 powders were synthesized from wet method and the DRX patterns showed that the crystallinity and particle size of CHA samples increased proportionally with the synthesis temperature. Powder extracts medium were obtained from each sample to interact with MC3T3-E1 osteoblastics cells. It was evaluated morphology, citotoxicity, pH and Ca²⁺ concentration. Citotoxicity assays showed high metabolic activity on all samples when compared to control. The polygonal shaped and the confluent monolayer observed in control cells progressively changed according to the crystallinity increase of samples. Cells under mitosis and spindle-like shaped where the main alterations observed. In addition the cell viability could be sensitive to the acid reactivity and crystallinity of carbonated apatite samples.

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Key Engineering Materials (Volumes 493-494)

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331-336

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.493-494.331

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Online since:

October 2011

Authors:

Elena Mavropoulos, Moema Hausen, Andrea Machado Costa, Silvia Raquel Albuquerque, Gutemberg Alves, J.M. Granjeiro, Alexandre Malta Rossi

Keywords:

Biocompatibility, Carbonated Apatite, Cytotoxicity, MC3T3

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