Effect of Silver Concentration on Bioactivity and Antibacterial Properties of SiO₂-CaO-P₂O₅ Sol-Gel Derived Bioactive Glass

Abstract:

Human beings are often infected by microorganisms in the living environment. Among various natural and inorganic substances, silver or silver ions have a powerful antibacterial activity. In the present study, the bioactivity and antibacterial activity of the SiO₂–CaO–P₂O₅–Ag₂O was compared with that of its ternary counterpart glass system (as a control sample) in vitro. The molar ratio of silver oxide in the bioactive glass composition was considered as different amount (0.5%, 1% and 2%). The surface characterization was evaluated after soaking in simulated body fluid (SBF). The sharpest apatite peak in X-ray diffraction (XRD) analyse after 7 days soaking in SBF was observed for 2% Ag sample (2%Ag-BG). Hence, the antibacterial effects of 2%Ag-BG sample against 2 gram negatives bacterium were examined by agar plate test. The result showed that the amount of silver did not prevent the HAp formation. Also, the antibacterial properties of 2%Ag-BG sample indicated near 100% bactericidal ratio (according to the width of antibacterial halo).

• Periodical: Key Engineering Materials (Volumes 493-494)
• Pages: 74-79
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.493-494.74
• Online since: October 2011
• Authors: Nader Nezafati, F. Moztarzadeh, Saeed Hesaraki, Masoud Mozafari, Ali Samadikuchaksaraei, Leila Hajibaki, Mazaher Gholipour
• Keywords: Agar Plate Test, Antibacterial Properties, Bioactive Glass, Gram Negatives Bacterium, Silver

References

[1] P. Tatar, N. Kiraz, M. Asilturk, F. Sayılkan, H. Sayılkan, E. Arpac¸ Antibacterial Thin Films on Glass Substrate by Sol–Gel Process. J. INORG. ORGANOMET. P., 17 (2007) 525-533.

DOI: 10.1007/s10904-007-9142-3

[2] S.M. Lee, B.S. Lee, T.G. Byun, K.C. Song, Preparation and antibacterial activity of silver-doped organic–inorganic hybrid coatings on glass substrates. Colloids and Surfaces A: Physicochem. Eng. Aspects 355 (2010) 167–171.

DOI: 10.1016/j.colsurfa.2009.12.010

[3] H.J. Jeon, S.C. Yi, S.G. Oh, Preparation and antibacterial effects of Ag–SiO2 thin films by sol–gel method. Biomaterials 24 (2003) 4921–4928.

DOI: 10.1016/s0142-9612(03)00415-0

[4] A. Sepahvandi, F. Moztarzadeh, M. Mozafari, M. Ghaffari, N. Raee, Photoluminescence in the characterization and early detection of biomimetic bone-like apatite formation on the surface of alkaline-treated titanium implant: State of the art. Colloids Surf B: Biointerfaces 86 (2011).

DOI: 10.1016/j.colsurfb.2011.04.027

[5] E. Vernè, S.D. Nunzio, M. Bosetti, P. Appendino, C.V. Brovarone, G. Maina, Surface characterization of silver-doped bioactive glass. Biomaterials 26 (2005) 5111-9.

DOI: 10.1016/j.biomaterials.2005.01.038

[6] N. Nezafati, F. Moztarzadeh, S. Hesaraki, M. Mozafari, Synergistically reinforcement of a self-setting calcium phosphate cement with bioactive glass fibers, Ceramics International, 37 (2010) 927-934.

DOI: 10.1016/j.ceramint.2010.11.002

[7] S. A. Poursamar, M. Azami, M. Mozafari, Controllable synthesis and characterization of porous polyvinyl alcohol/hydroxyapatite nanocomposite scaffolds via an in situ colloidal technique, Colloids Surf. B: Biointerfaces, 84 (2011) 310-316.

DOI: 10.1016/j.colsurfb.2011.01.015

[8] A. Hamlekhan, M. Mozafari, N. Nezafati, M. Azami, H. Hadipour, A proposed fabrication method of novel PCL-GEL-HAp nanocomposite scaffolds for bone tissue engineering applications, Advanced Composites Letters, 19 (2010), 123-130.

DOI: 10.1177/096369351001900401

[9] M. Azami, S. Jalilifiroozinezhad, M. Mozafari, Synthesis and solubility of calcium fluoride/hydroxy-fluorapatite nanocrystals for dental applications, Ceramics International 37 (2011) 2007-(2014).

DOI: 10.1016/j.ceramint.2011.02.025

[10] M. Mozafari, M. Rabiee, M. Azami, S. Maleknia, Biomimetic formation of apatite on the surface of porous gelatin/bioactive glass nanocomposite scaffolds, Applied Surface Science 257 (2010), 1740-1749.

DOI: 10.1016/j.apsusc.2010.09.008

[11] A. Balamurugan, G. Balossier, D. Laurent-Maquin, S. Pina, A.H.S. Rebelo, J. Faure, J.M.F. Ferreira, An in vitro biological and anti-bacterial study on a sol–gel derived silver-incorporated bioglass system. Dent Mater 24 (2008) 1343-51.

DOI: 10.1016/j.dental.2008.02.015

[12] M.R. Filgueiras, G.P. Latorre, L.L. Hench, Solution effects on thesurface reaction of a bioactive glass. J. Biomed. Mater. Res. 27 (1993) 445–53.

[13] S. Efrima, B.V. Bronk, Silver colloids and impregnating or coating bacteria. J. Phys. Chem. B. 102 (1998) 5947–50.

DOI: 10.1021/jp9813903

[14] S.Y. Liaue, D.C. Read, W.J. Pugh, J.R. Furr, A.D. Russel, Interaction of silver nitrate with readily identifiable groups; relationship to the antibacterial action of the silver ions. Lett. Appl. Microbiol. 25 (1997) 279–83.

DOI: 10.1046/j.1472-765x.1997.00219.x

[15] M. Solioz, A. Odermatt, Copper and silver transport by CopB-ATPase in membrane vesicles of Entrococcus hirae. J. Biol. Chem. 270 (1995) 9217–21.

DOI: 10.1074/jbc.270.16.9217

[16] T.N. Wells, P. Scully, G. Paravinchini, A.E. Proudfoot, M.A. Payton, Mechanism of irreversible inactivation of phosphmannose isomerase by silver ions and famazine. Biochemistry 34 (1995) 7896–903.

DOI: 10.1021/bi00024a014