Calcium Phosphate Coated Hydroxyapatite/Collagen Nanocomposite Membrane for Surface-Mediated Gene Transfer

Abstract:

Calcium phosphate (CaP) coating is an effective technique for surface functionalization of biomaterials. The objective of our research is to prepare calcium phosphate (CaP) coatings on a hydroxyapatite/collagen (HAp/Col) nanocomposite and subsequently provide it with gene delivery function through the immobilization of DNA in the coating. We have specifically selected the HAp/Col nanocomposite since it has the high potential as bone substitutes due to its similar composition, nanostructure, and biological properties to those of human bone. CaP coatings consisting of different sized particles were prepared on the HAp/Col nanocomposite membrane by immersing it in supersaturaterd CaP solutions (so-called RKM solutions) with the varied Ca and P concentration levels. We immobilized DNA in the CaP coatings together with lipid and fibronectin by supplementing DNA, lipid, and fibronectin to the RKM solutions (DLF-RKM solutions). Gene transfer capability of the resulting HAp/Col nanocomposite membrane was improved with decreasing concentration level of the DLF-RKM solution. It was confirmed that the present CaP coating technique was effective in providing the HAp/Col nanocomposite membrane with gene transfer capability and that the Ca and P concentration level of the DLF-RKM solution was a controlling factor affecting the gene transfer efficiency.

• Periodical: Key Engineering Materials (Volumes 529-530)
• Pages: 490-494
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.529-530.490
• Online since: November 2012
• Authors: Subhadip Bodhak, Masanori Kikuchi, Ayako Oyane, Yu Sogo, Hideo Tsurushima, Atsuo Ito
• Keywords: Biomimetic Process, Calcium Phosphate Coating, Gene Transfer, Hydroxyapatite/Collagen Nanocomposite Membrane, Supersaturated Solution

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