Novel Apatite-Pathogen-Associated Molecular Patterns Adjuvants for Cancer Immune Therapy

Abstract:

An apatite-pathogen-associated molecular patterns (PAMPs) adjuvant was prepared by coprecipitation of apatite and PAMPs using a supersaturated calcium phosphate solution supplemented with PAMPs. In this study, we used a hydrothermal extract of human tubercle bacillus (HTB) as PAMPs. The effects of coprecipitation conditions on immunogenic activity were studied. The adjuvants prepared using extract of at 10 and 20 μg/mL showed higher in vitro immunogenic activity than that at 2 μg/mL. The adjuvants prepared under stir at speed of 300 rpm showed better in vitro immunogenic activity than those under still. The adjuvants prepared using 10 μg/mL of extract of HTB under stir at speed of 300 rpm may be promising for cancer immune therapy.

• Periodical: Key Engineering Materials (Volumes 529-530)
• Pages: 471-474
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.529-530.471
• Online since: November 2012
• Authors: Xiu Peng Wang, Xia Li, Atsuo Ito, Yu Sogo
• Keywords: Adjuvant, Apatite, Cancer Immune, Pathogen-Associated Molecular Patterns

References

[1] H. Oka, Y. Emori, O. Ohya, N. Kobayashi, H. Sasaki, Y. Tanaka, Y. Hayashi, K. Nomoto, An immunomodulatory arabinomannan extracted from Mycobacterium tuberculosis, Z-100, restores the balance of Th1/Th2 cell responses in tumor bearing mice, Immunol Lett. 70 (1999).

DOI: 10.1016/s0165-2478(99)00130-3

[2] H. Oka, H. Sasaki, Y. Shiraishi, Y. Emori, K. Yoshinaga, M. Takei, Z-100, an immunomodulatory arabinomannan extracted from Mycobacterium tuberculosis strain aoyama B, augments anti-tumor activities of X-ray irradiation against B16 melanoma in association with the improvement of type 1 T cell responses, Biol Pharm Bull. 27 (2004).

DOI: 10.1248/bpb.27.82

[3] H. Sasaki, D. Schmitt, Y. Hayashi, R.B. Pollard, F. Suzuki, Antitumor Mechanisms of Z-100, an Immunomodulatory Arabinomannan Extracted from Mycobacterium-Tuberculosis - the Importance of Lymphocytes Infiltrated into Tumor Sites, Nat Immun. 12 (1993).

[4] M. Kobayashi, R.B. Pollard, F. Suzuki, Inhibition of Lung Metastases of B16 Melanoma by an Immunomodulatory Arabinomannan Extracted from Mycobacterium-Tuberculosis (Z-100), J Immunol. 150 (1993) A309-A309.

DOI: 10.1097/00001813-199702000-00006

[5] M. Kobayashi, R.B. Pollard, F. Suzuki, Inhibition of pulmonary metastasis by Z-100, an immunomodulatory lipid-arabinomannan extracted from Mycobacterium tuberculosis, in mice inoculated with B16 melanoma, Anti-Cancer Drug. 8 (1997) 156-163.

DOI: 10.1097/00001813-199702000-00006

[6] S.C. Eisenbarth, O.R. Colegio, W. O'Connor, F.S. Sutterwala, R.A. Flavell, Crucial role for the Nalp3 inflammasome in the immunostimulatory properties of aluminium adjuvants, Nature. 453 (2008) 1122-U1113.

DOI: 10.1038/nature06939

[7] O.A. Ali, N. Huebsch, L. Cao, G. Dranoff, D.J. Mooney, Infection-mimicking materials to program dendritic cells in situ, Nature Materials. 8 (2009) 151-158.

DOI: 10.1038/nmat2357

[8] X. Li, X. Wang, A. Ito, Y. Sogo, K. Cheng, A. Oyane, Effect of Coprecipitation Temperature on the Properties and Activity of Fibroblast Growth Factor-2 Apatite Composite Layer, Materials Science and Engineering C. 29 (2008) 216-221.

DOI: 10.1016/j.msec.2008.06.012

[9] X. Wang, A. Ito, Y. Sogo, X. Li, H. Tsurushima, A. Oyane, Ascorbate-apatite composite and ascorbate-FGF-2-apatite composite layers formed on external fixation rods and their effects on cell activity in vitro, Acta Biomater. 5 (2009) 2647-2656.

DOI: 10.1016/j.actbio.2009.03.020

[10] X. Wang, A. Ito, Y. Sogo, X. Li, A. Oyane, Zinc-containing apatite layers on external fixation rods promoting cell activity, Acta biomaterialia. 6 (2010) 962-968.

DOI: 10.1016/j.actbio.2009.08.038

[11] X.P. Wang, X. Li, K. Onuma, A. Ito, Y. Sogo, K. Kosuge, A. Oyane, Mesoporous bioactive glass coatings on stainless steel for enhanced cell activity, cytoskeletal organization and AsMg immobilization, J Mater Chem. 20 (2010) 6437-6445.

DOI: 10.1039/c0jm00399a

[12] X.P. Wang, A. Ito, Y. Sogo, X. Li, A. Oyane, Silicate-apatite composite layers on external fixation rods and in vitro evaluation using fibroblast and osteoblast, J Biomed Mater Res A. 92A (2010) 1181-1189.

DOI: 10.1002/jbm.a.32436

[13] X. Wang, A. Ito, X. Li, Y. Sogo, A. Oyane, Signal molecules-calcium phosphate coprecipitation and its biomedical application as a functional coating, Biofabrication. 3 (2011) 022001.

DOI: 10.1088/1758-5082/3/2/022001

[14] Y. Shi, C.H. Liu, A.I. Roberts, J. Das, G. Xu, G. Ren, Y. Zhang, L. Zhang, Z.R. Yuan, H.S. Tan, G. Das, S. Devadas, Granulocyte-macrophage colony-stimulating factor (GM-CSF) and T-cell responses: what we do and don't know, Cell Res. 16 (2006).

DOI: 10.1038/sj.cr.7310017

[15] J. Dou, Y. Wang, J. Wang, F. Zhao, Y. Li, M. Cao, W. Hu, K. Hu, X. He, L. Chu, C. Jiang, N. Gu, Antitumor efficacy induced by human ovarian cancer cells secreting IL-21 alone or combination with GM-CSF cytokines in nude mice model, Immunobiology. 214 (2009).

DOI: 10.1016/j.imbio.2008.11.002

[16] N.L. Berinstein, Enhancing cancer vaccines with immunomodulators, Vaccine. 25S (2007) B72-B88.

DOI: 10.1016/j.vaccine.2007.06.043