Adsorption Behavior of Albumin and other Proteins on Carbon Nanotubes Studied by Chromatography

Abstract:

For application of carbon nanotubes (CNTs) as biomaterials, it is important to clarify the interaction between CNTs and proteins, which may affect on cell proliferation and differentiation. In this study, the adsorption behavior was investigated for representative proteins, bovine serum albumin, lysozyme and fetal bovine serum by using chromatography system. It was also aimed whether the different treatment conditions of CNTs affected the adsorption of proteins. CNTs used for this study had a straight shape and about 70nm in a diameter from SEM observation. There was not much difference between untreated and treated CNTs from SEM images. In chromatography experiments, all the proteins of albumin, lysozyme and serum were eluted immediately after injected to the column of untreated CNTs. Second elution appeared after buffer was changed from phosphate saline buffer to 25mM sodium hydroxide (NaOH). The same tendency was confirmed for CNTs with only acid treatment. However the eluted peaks became remarkably smaller after the column was changed to CNTs with thermal and twice acid treatments. These results indicated that treatment conditions for CNTs affected the adsorption behavior of proteins.

• Periodical: Key Engineering Materials (Volumes 529-530)
• Pages: 615-620
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.529-530.615
• Online since: November 2012
• Authors: M. Morikawa, Y. Kuboki, Tsukasa Akasaka, Shigeaki Abe, H. Takita, Fumio Watari
• Keywords: Adsorption, Carbon Nanotube (CN), Chromatography, Protein, Purification Treatment

References

[1] J.P. Gleeson, N.A. Plunkett, F.J. O'Brien, Tissue engineering tools for modulation of the immune response, Bio Techniques. 51 (2011) 239-254.

[2] S. Iijima, Helical microtubules of graphitic carbon, Nature. 354 (1991) 56-58.

DOI: 10.1038/354056a0

[3] N. Sinha, JT. Yeow, Carbon nanotubes for biomedical applications, IEEE Trans Nanobioscience. 4 (2005) 180-195.

DOI: 10.1109/tnb.2005.850478

[4] M. Uo, K. Tamura, Y. Sato, A. Yokoyama, F. Watari, Y. Totsuka, K. Thoji, Cytotoxicity of metalencapsulating carbon nanocapsules, Small. 1 (2005) 816-819.

DOI: 10.1002/smll.200400143

[5] N. Aoki, A. Yokoyama, Y. Nodasaka, T. Akasaka, M. Uo, Y. Sato, K. Tohji, F. Watari, Cell culture on a carbon nanotube scaffold, J. Biomed. Nanotechnol. 1 (2005) 402-405.

DOI: 10.1166/jbn.2005.048

[6] N. Aoki, A. Yokoyama, Y. Nodasaka, T. Akasaka, M. Uo, Y. Sato, K. Tohji, F. Watari, Strinkgly extended morphology of cells grown on carbon nanotubes, Chem Lett. 35 (2006) 508-509.

DOI: 10.1246/cl.2006.508

[7] X. Li, H. Liu, X. Niu, B. Yu, Y. Fan, Q. Feng, FZ. Cui, F. Watari, The use of carbon nanotubes to induce osteogenic differentiation of human adipose-derived MSCs in vitro and ectopic bone formation invitro, Biomaterials. 33 (2012) 4818-4827.

DOI: 10.1016/j.biomaterials.2012.03.045

[8] N. Aoki, T. Akasaka, F. Watari, A. Yokoyama, Carbon nanotubes as scaffolds for cell culture and effect on cellularfunctions, Dent. Mat.J. 26 (2007) 178-185.

DOI: 10.4012/dmj.26.178

[9] TJ. Webster, MC. Waid, JL. Mckenzie, RL. Price, JU. Ejiofor, Nano-biotechnology: carbon nanofibers as improved neural and orthopedic implants, Nanotechnology. 15 (2005) 48-54.

DOI: 10.1088/0957-4484/15/1/009

[10] A. Abarratetegi, MC. Gutiérrez, C. Moreno-Vincent, MJ. Hortigüela, V. Ramos, JL. López-Lacomba, ML. Ferrer, F. del Monte, Multiwall carbon nanotubes scaffolds foe tissue engineering purpose, Biomaterials. 29 (2008) 94-102.

DOI: 10.1016/j.biomaterials.2007.09.021

[11] LE. Valenti, PA. Fiorito, CD. Garcia, CE. Giacomelli, The adsorption-desorption process of bovine serum albumin on carbon nanotubes, J. Colloid Interface Sci. 307 (2007) 349-356.

DOI: 10.1016/j.jcis.2006.11.046

[12] Y. Kuboki, T. Koshikawa, H. Takita, R. Fujisawa, MH. Lee, S. Abe, T. Akasaka, F. Watari, R. Sammons, Carbon nanotubes chromatography separated albumin from other serum proteins: a method for direct analysis of their interactions, Dent. Mat.J. 29 (2010).

DOI: 10.4012/dmj.2009-097

[13] Y. Sato, A. Yokoyama, K. Shibata, Y. Akimoto, S. Ogino, Y. Nodasaka, T. Kohgo, K. Tamura , T. Akasaka, M. Uo, K. Motomiya, B. Jeyadevan, M. Ishiguro, R. Hatakeyama, F. Watari, K. Tohji , Influence of length on cytotoxity of multi-walled carbon nanotubes against human acute monocytic leukemia cell line THP-1 in vitro and subcutaneous tissue of rats in vivo, Mol Biosyst. 1 (2005).

DOI: 10.1039/b502429c

[14] H. Peng, LB. Alemany, JL. Margrave, VN. Khabashesku, Sidewall carboxylic acid functionalization of single-walled carbon nanotubes, J. Am. Chem. Soc. 125 (2003) 15174-15182.

DOI: 10.1021/ja037746s

[15] Z. Du, YL. Yu, XW. Chen, JH. Wang, The isolation of basic proteins by solid-phase extraction with multiwalled carbon nanotubes. Chem Eur J 2007; 13: 9679-9.

DOI: 10.1002/chem.200700784